@article{a6964e6f6ab048ab83dd63cdd67ec2b6,
title = "How do we use in vitro models to understand epileptiform and ictal activity? A report of the TASK1-WG4 group of the ILAE/AES Joint Translational Task Force",
abstract = "In vitro brain tissue preparations allow the convenient and affordable study of brain networks and have allowed us to garner molecular, cellular, and electrophysiologic insights into brain function with a detail not achievable in vivo. Preparations from both rodent and human postsurgical tissue have been utilized to generate in vitro electrical activity similar to electrographic activity seen in patients with epilepsy. A great deal of knowledge about how brain networks generate various forms of epileptiform activity has been gained, but due to the multiple in vitro models and manipulations used, there is a need for a standardization across studies. Here, we describe epileptiform patterns generated using in vitro brain preparations, focusing on issues and best practices pertaining to recording, reporting, and interpretation of the electrophysiologic patterns observed. We also discuss criteria for defining in vitro seizure-like patterns (i.e., ictal) and interictal discharges. Unifying terminologies and definitions are proposed. We suggest a set of best practices for reporting in vitro studies to favor both efficient across-lab comparisons and translation to in vivo models and human studies.",
keywords = "Ictal activity, In vitro models, Review",
author = "Dulla, {Chris G.} and Damir Janigro and Premysl Jiruska and Raimondo, {Joseph V.} and Akio Ikeda and Lin, {Chou Ching K.} and Goodkin, {Howard P.} and Galanopoulou, {Aristea S.} and Christophe Bernard and {de Curtis}, Marco",
note = "Funding Information: ASG has received royalties for publications from Elsevier and is a co-Editor-in-Chief of Epilepsia Open. ASG has received a one-time honorarium for participation at a scientific advisory board for Mallinckrodt. AI is a member of the Department of Epilepsy, Movement Disorders, and Physiology, Kyoto University Graduate School of Medicine, which is an endowment department supported with a grant from GlaxoSmithKline K.K., Nihon Kohden Corporation, Otsuka Pharmaceutical Co., and UCB Japan Co. The remaining authors have no conflicts of interest to disclose. We confirm that we have read the Journal{\textquoteright}s position on issues involved in ethical publication and affirm that this report is consistent with those guidelines. Funding Information: The authors would like to thank Dr. Uwe Heinemann, who sadly passed away in 2016, for his early discussions within our working group as well as his past scientific contributions that are highlighted in this review. Uwe was a true leader in the field of epilepsy research. We would also like to acknowledge Lauren-Harte-Hargrove for assistance during the preparation of this manuscript. This report was written by experts selected by the International League Against Epilepsy (ILAE) and the American Epilepsy Society (AES) and was approved for publication by the ILAE and the AES. Opinions expressed by the authors, however, do not necessarily represent the policy or position of the ILAE or the AES. We are grateful to the AES and ILAE for partial sponsoring the activities of the ILAE/AES Joint Translational Task Force. The authors have received partial travel reimbursement to attend some of the meetings of the TASK1 group of the ILAE/AES Joint Translational Task Force through the AES and the ILAE. CGD acknowledges grant support by National Institute of Neurological Disease and Stroke, Department of Defense, the Citizens United for Research in Epilepsy Infantile Spasms Initiative, and Tufts University. ASG acknowledges grant support by NINDS RO1 NS091170, U54 NS100064, the United States Department of Defense (W81XWH-13-1-0180), the CURE Infantile Spasms Initiative, and research funding from the Heffer Family, the Segal Family Foundations, and the Abbe Goldstein/Joshua Lurie and Laurie Marsh/Dan Levitz families. PJ acknowledges grant support by the Ministry of Health of the Czech Republic (AZV15-29835A, AZV17-28427A, AZV15-33115A) and Czech Science Foundation (18-07908S). Funding Information: The authors would like to thank Dr. Uwe Heinemann, who sadly passed away in 2016, for his early discussions within our working group as well as his past scientific contributions that are highlighted in this review. Uwe was a true leader in the field of epilepsy research. We would also like to acknowledge Lauren-Harte-Hargrove for assistance during the preparation of this manuscript. This report was written by experts selected by the International League Against Epilepsy (ILAE) and the American Epilepsy Society (AES) and was approved for publication by the ILAE and the AES. Opinions expressed by the authors, however, do not necessarily represent the policy or position of the ILAE or the AES. We are grateful to the AES and ILAE for partial sponsoring the activities of the ILAE/AES Joint Translational Task Force. The authors have received partial travel reimbursement to attend some of the meetings of the TASK1 group of the ILAE/AES Joint Translational Task Force through the AES and the ILAE. CGD acknowledges grant support by National Institute of Neurological Disease and Stroke, Department of Defense, the Citizens United for Research in Epilepsy Infantile Spasms Initiative, and Tufts University. ASG acknowledges grant support by NINDS RO1 NS091170, U54 NS100064, the United States Department of Defense (W81XWH-13-1-0180), the CURE Infantile Spasms Initiative, and research funding from the Heffer Family, the Segal Family Foundations, and the Abbe Goldstein/Joshua Lurie and Laurie Marsh/Dan Levitz families. PJ acknowledges grant support by the Ministry of Health of the Czech Republic (AZV15-29835A, AZV17-28427A, AZV15-33115A) and Czech Science Foundation (18-07908S) . Publisher Copyright: {\textcopyright} 2018 The Authors. Epilepsia Open published by Wiley Periodicals Inc. on behalf of International League Against Epilepsy.",
year = "2018",
month = dec,
doi = "10.1002/epi4.12277",
language = "English (US)",
volume = "3",
pages = "460--473",
journal = "Epilepsia Open",
issn = "2470-9239",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "4",
}