Abstract
In vitro brain tissue preparations allow the convenient and affordable study of brain networks and have allowed us to garner molecular, cellular, and electrophysiologic insights into brain function with a detail not achievable in vivo. Preparations from both rodent and human postsurgical tissue have been utilized to generate in vitro electrical activity similar to electrographic activity seen in patients with epilepsy. A great deal of knowledge about how brain networks generate various forms of epileptiform activity has been gained, but due to the multiple in vitro models and manipulations used, there is a need for a standardization across studies. Here, we describe epileptiform patterns generated using in vitro brain preparations, focusing on issues and best practices pertaining to recording, reporting, and interpretation of the electrophysiologic patterns observed. We also discuss criteria for defining in vitro seizure-like patterns (i.e., ictal) and interictal discharges. Unifying terminologies and definitions are proposed. We suggest a set of best practices for reporting in vitro studies to favor both efficient across-lab comparisons and translation to in vivo models and human studies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 460-473 |
| Number of pages | 14 |
| Journal | Epilepsia Open |
| Volume | 3 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 1 2018 |
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Keywords
- Ictal activity
- In vitro models
- Review
ASJC Scopus subject areas
- Clinical Neurology
- Neurology
Cite this
How do we use in vitro models to understand epileptiform and ictal activity? A report of the TASK1-WG4 group of the ILAE/AES Joint Translational Task Force. / Dulla, Chris G.; Janigro, Damir; Jiruska, Premysl; Raimondo, Joseph V.; Ikeda, Akio; Lin, Chou Ching K.; Goodkin, Howard P.; Galanopoulou, Aristea S.; Bernard, Christophe; de Curtis, Marco.
In: Epilepsia Open, Vol. 3, No. 4, 01.12.2018, p. 460-473.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - How do we use in vitro models to understand epileptiform and ictal activity? A report of the TASK1-WG4 group of the ILAE/AES Joint Translational Task Force
AU - Dulla, Chris G.
AU - Janigro, Damir
AU - Jiruska, Premysl
AU - Raimondo, Joseph V.
AU - Ikeda, Akio
AU - Lin, Chou Ching K.
AU - Goodkin, Howard P.
AU - Galanopoulou, Aristea S.
AU - Bernard, Christophe
AU - de Curtis, Marco
PY - 2018/12/1
Y1 - 2018/12/1
N2 - In vitro brain tissue preparations allow the convenient and affordable study of brain networks and have allowed us to garner molecular, cellular, and electrophysiologic insights into brain function with a detail not achievable in vivo. Preparations from both rodent and human postsurgical tissue have been utilized to generate in vitro electrical activity similar to electrographic activity seen in patients with epilepsy. A great deal of knowledge about how brain networks generate various forms of epileptiform activity has been gained, but due to the multiple in vitro models and manipulations used, there is a need for a standardization across studies. Here, we describe epileptiform patterns generated using in vitro brain preparations, focusing on issues and best practices pertaining to recording, reporting, and interpretation of the electrophysiologic patterns observed. We also discuss criteria for defining in vitro seizure-like patterns (i.e., ictal) and interictal discharges. Unifying terminologies and definitions are proposed. We suggest a set of best practices for reporting in vitro studies to favor both efficient across-lab comparisons and translation to in vivo models and human studies.
AB - In vitro brain tissue preparations allow the convenient and affordable study of brain networks and have allowed us to garner molecular, cellular, and electrophysiologic insights into brain function with a detail not achievable in vivo. Preparations from both rodent and human postsurgical tissue have been utilized to generate in vitro electrical activity similar to electrographic activity seen in patients with epilepsy. A great deal of knowledge about how brain networks generate various forms of epileptiform activity has been gained, but due to the multiple in vitro models and manipulations used, there is a need for a standardization across studies. Here, we describe epileptiform patterns generated using in vitro brain preparations, focusing on issues and best practices pertaining to recording, reporting, and interpretation of the electrophysiologic patterns observed. We also discuss criteria for defining in vitro seizure-like patterns (i.e., ictal) and interictal discharges. Unifying terminologies and definitions are proposed. We suggest a set of best practices for reporting in vitro studies to favor both efficient across-lab comparisons and translation to in vivo models and human studies.
KW - Ictal activity
KW - In vitro models
KW - Review
UR - http://www.scopus.com/inward/record.url?scp=85061337019&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85061337019&partnerID=8YFLogxK
U2 - 10.1002/epi4.12277
DO - 10.1002/epi4.12277
M3 - Article
AN - SCOPUS:85061337019
VL - 3
SP - 460
EP - 473
JO - Epilepsia Open
JF - Epilepsia Open
SN - 2470-9239
IS - 4
ER -