How actin crosslinking and bundling proteins cooperate to generate an enhanced cell mechanical response

Yiider Tseng, Thomas P. Kole, Jerry S H Lee, Elena Fedorov, Steven C. Almo, Benjamin W. Schafer, Denis Wirtz

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Actin-crosslinking proteins organize actin filaments into dynamic and complex subcellular scaffolds that orchestrate important mechanical functions, including cell motility and adhesion. Recent mutation studies have shown that individual crosslinking proteins often play seemingly non-essential roles, leading to the hypothesis that they have considerable redundancy in function. We report live-cell, in vitro, and theoretical studies testing the mechanical role of the two ubiquitous actin-crosslinking proteins, α-actinin and fascin, which co-localize to stress fibers and the basis of filopodia. Using live-cell particle tracking microrheology, we show that the addition of α-actinin and fascin elicits a cell mechanical response that is significantly greater than that originated by α-actinin or fascin alone. These live-cell measurements are supported by quantitative rheological measurements with reconstituted actin filament networks containing pure proteins that show that α-actinin and fascin can work in concert to generate enhanced cell stiffness. Computational simulations using finite element modeling qualitatively reproduce and explain the functional synergy of α-actinin and fascin. These findings highlight the cooperative activity of fascin and α-actinin and provide a strong rationale that an evolutionary advantage might be conferred by the cooperative action of multiple actin-crosslinking proteins with overlapping but non-identical biochemical properties. Thus the combination of structural proteins with similar function can provide the cell with unique properties that are required for biologically optimal responses.

Original languageEnglish (US)
Pages (from-to)183-192
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume334
Issue number1
DOIs
StatePublished - Aug 19 2005

Fingerprint

Actinin
Crosslinking
Actins
Proteins
Actin Cytoskeleton
Cell Tracking
Stress Fibers
Pseudopodia
Cell Adhesion
Scaffolds
Cell Movement
Redundancy
fascin
Theoretical Models
Adhesion
Stiffness
Mutation
Fibers
Testing

Keywords

  • α-Actinin
  • Actin
  • Cell mechanics
  • Cytoskeleton
  • Fascin
  • Multiple-particle tracking microrheology

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

How actin crosslinking and bundling proteins cooperate to generate an enhanced cell mechanical response. / Tseng, Yiider; Kole, Thomas P.; Lee, Jerry S H; Fedorov, Elena; Almo, Steven C.; Schafer, Benjamin W.; Wirtz, Denis.

In: Biochemical and Biophysical Research Communications, Vol. 334, No. 1, 19.08.2005, p. 183-192.

Research output: Contribution to journalArticle

Tseng, Yiider ; Kole, Thomas P. ; Lee, Jerry S H ; Fedorov, Elena ; Almo, Steven C. ; Schafer, Benjamin W. ; Wirtz, Denis. / How actin crosslinking and bundling proteins cooperate to generate an enhanced cell mechanical response. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 334, No. 1. pp. 183-192.
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