Hormone-sensitive adenylate cyclase in glomerular cells possible role for inflammatory diseases of the glomerulus

Using the adenylate cyclase assay after Ross we examined hormone sensitivity of isolated glomerular cells. cAMP production was increased 1.3–1.6-fold by stimulation with isoproterenol, 1.5–1.8 times by prostaglandin E₁ and 1.4–1.5 times by histamine. The isoproterenol reaction could be completely inhibited by propranolol, the histamine effect was abolished by the H2-blocking agent cimetidine. As control we applied sodium fluoride, which directly activates the catalytic adenylate cyclase unit, increasing the activity 1.8–2.7 times (depending on the method of homogenization). These findings could reflect some physiological or pathophysiological implications, which are discussed in the present report.