TY - JOUR
T1 - HLA-DR expression in B-cell non-Hodgkin's malignant lymphomas. A multiparameter flow cytometry study
AU - Ratech, Howard
N1 - Funding Information:
From the Fox Chase Cancer Center, Department of Pathology, Philadelphia, PA. Accepted for publication April 3, 1990. Dr Ratech is currently affiliated with Columbia University, College of Physicians and Surgeons, Department of Pathology, New York, NY. Supported by Grant No. CA06927 from the National Institutes of Health, Bethesda, MD, and an appropriation from the
PY - 1990/12
Y1 - 1990/12
N2 - Ten cases of reactive follicular hyperplasia and 31 cases of B-cell non-Hodgkin's malignant lymphoma were studied using multiparameter flow cytometry. A bimodal distribution for HLA-DR expression, but not for surface immunoglobulin or B cell-specific antigens CD19 and CD20, was observed commonly in mixed cell type and infrequently in non-mixed cell type B-cell malignant lymphomas. On the basis of HLA-DR distribution alone, 31 case of B-cell malignant lymphomas of low, intermediate, and high grades could be separated into mixed and non-mixed cell types, with only two misclassifications (P=0.0001). Exceptionally, one case of malignant lymphoma, follicular and diffuse, mixed-cell type had a unimodal HLA-DR distribution, and one case of malignant lymphoma, diffuse, large noncleaved cell type had a bimodal HLA-DR distribution. In all cases of malignant lymphoma, follicular, mixed-cell type studied, low HLA-DR was correlated with small cells, and high HLA-DR was correlated with large cells. In contrast, HLA-DR expression and cell size were not as directly correlated in cases of malignant lymphoma, diffuse, mixed-cell type. These observations suggest that most, but not all, cases of B-cell malignant lymphomas of the mixed cell type can be separated from other B-cell lymphomas on the basis of HLA-DR distribution.
AB - Ten cases of reactive follicular hyperplasia and 31 cases of B-cell non-Hodgkin's malignant lymphoma were studied using multiparameter flow cytometry. A bimodal distribution for HLA-DR expression, but not for surface immunoglobulin or B cell-specific antigens CD19 and CD20, was observed commonly in mixed cell type and infrequently in non-mixed cell type B-cell malignant lymphomas. On the basis of HLA-DR distribution alone, 31 case of B-cell malignant lymphomas of low, intermediate, and high grades could be separated into mixed and non-mixed cell types, with only two misclassifications (P=0.0001). Exceptionally, one case of malignant lymphoma, follicular and diffuse, mixed-cell type had a unimodal HLA-DR distribution, and one case of malignant lymphoma, diffuse, large noncleaved cell type had a bimodal HLA-DR distribution. In all cases of malignant lymphoma, follicular, mixed-cell type studied, low HLA-DR was correlated with small cells, and high HLA-DR was correlated with large cells. In contrast, HLA-DR expression and cell size were not as directly correlated in cases of malignant lymphoma, diffuse, mixed-cell type. These observations suggest that most, but not all, cases of B-cell malignant lymphomas of the mixed cell type can be separated from other B-cell lymphomas on the basis of HLA-DR distribution.
KW - B cell
KW - HLA-DR
KW - flow cytometry
KW - major histocompatibility complex class II
KW - malignant lymphoma
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U2 - 10.1016/S0046-8177(06)80042-0
DO - 10.1016/S0046-8177(06)80042-0
M3 - Article
C2 - 2249840
AN - SCOPUS:0025678357
SN - 0046-8177
VL - 21
SP - 1275
EP - 1282
JO - Human Pathology
JF - Human Pathology
IS - 12
ER -