HLA-DR expression in B-cell non-Hodgkin's malignant lymphomas. A multiparameter flow cytometry study

Howard Ratech

Research output: Contribution to journalArticle

3 Scopus citations


Ten cases of reactive follicular hyperplasia and 31 cases of B-cell non-Hodgkin's malignant lymphoma were studied using multiparameter flow cytometry. A bimodal distribution for HLA-DR expression, but not for surface immunoglobulin or B cell-specific antigens CD19 and CD20, was observed commonly in mixed cell type and infrequently in non-mixed cell type B-cell malignant lymphomas. On the basis of HLA-DR distribution alone, 31 case of B-cell malignant lymphomas of low, intermediate, and high grades could be separated into mixed and non-mixed cell types, with only two misclassifications (P=0.0001). Exceptionally, one case of malignant lymphoma, follicular and diffuse, mixed-cell type had a unimodal HLA-DR distribution, and one case of malignant lymphoma, diffuse, large noncleaved cell type had a bimodal HLA-DR distribution. In all cases of malignant lymphoma, follicular, mixed-cell type studied, low HLA-DR was correlated with small cells, and high HLA-DR was correlated with large cells. In contrast, HLA-DR expression and cell size were not as directly correlated in cases of malignant lymphoma, diffuse, mixed-cell type. These observations suggest that most, but not all, cases of B-cell malignant lymphomas of the mixed cell type can be separated from other B-cell lymphomas on the basis of HLA-DR distribution.

Original languageEnglish (US)
Pages (from-to)1275-1282
Number of pages8
JournalHuman Pathology
Issue number12
Publication statusPublished - Dec 1990



  • B cell
  • HLA-DR
  • flow cytometry
  • major histocompatibility complex class II
  • malignant lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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