Abstract
Ten cases of reactive follicular hyperplasia and 31 cases of B-cell non-Hodgkin's malignant lymphoma were studied using multiparameter flow cytometry. A bimodal distribution for HLA-DR expression, but not for surface immunoglobulin or B cell-specific antigens CD19 and CD20, was observed commonly in mixed cell type and infrequently in non-mixed cell type B-cell malignant lymphomas. On the basis of HLA-DR distribution alone, 31 case of B-cell malignant lymphomas of low, intermediate, and high grades could be separated into mixed and non-mixed cell types, with only two misclassifications (P=0.0001). Exceptionally, one case of malignant lymphoma, follicular and diffuse, mixed-cell type had a unimodal HLA-DR distribution, and one case of malignant lymphoma, diffuse, large noncleaved cell type had a bimodal HLA-DR distribution. In all cases of malignant lymphoma, follicular, mixed-cell type studied, low HLA-DR was correlated with small cells, and high HLA-DR was correlated with large cells. In contrast, HLA-DR expression and cell size were not as directly correlated in cases of malignant lymphoma, diffuse, mixed-cell type. These observations suggest that most, but not all, cases of B-cell malignant lymphomas of the mixed cell type can be separated from other B-cell lymphomas on the basis of HLA-DR distribution.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1275-1282 |
| Number of pages | 8 |
| Journal | Human Pathology |
| Volume | 21 |
| Issue number | 12 |
| DOIs | |
| State | Published - 1990 |
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Keywords
- B cell
- flow cytometry
- HLA-DR
- major histocompatibility complex class II
- malignant lymphoma
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
HLA-DR expression in B-cell non-Hodgkin's malignant lymphomas. A multiparameter flow cytometry study. / Ratech, Howard.
In: Human Pathology, Vol. 21, No. 12, 1990, p. 1275-1282.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - HLA-DR expression in B-cell non-Hodgkin's malignant lymphomas. A multiparameter flow cytometry study
AU - Ratech, Howard
PY - 1990
Y1 - 1990
N2 - Ten cases of reactive follicular hyperplasia and 31 cases of B-cell non-Hodgkin's malignant lymphoma were studied using multiparameter flow cytometry. A bimodal distribution for HLA-DR expression, but not for surface immunoglobulin or B cell-specific antigens CD19 and CD20, was observed commonly in mixed cell type and infrequently in non-mixed cell type B-cell malignant lymphomas. On the basis of HLA-DR distribution alone, 31 case of B-cell malignant lymphomas of low, intermediate, and high grades could be separated into mixed and non-mixed cell types, with only two misclassifications (P=0.0001). Exceptionally, one case of malignant lymphoma, follicular and diffuse, mixed-cell type had a unimodal HLA-DR distribution, and one case of malignant lymphoma, diffuse, large noncleaved cell type had a bimodal HLA-DR distribution. In all cases of malignant lymphoma, follicular, mixed-cell type studied, low HLA-DR was correlated with small cells, and high HLA-DR was correlated with large cells. In contrast, HLA-DR expression and cell size were not as directly correlated in cases of malignant lymphoma, diffuse, mixed-cell type. These observations suggest that most, but not all, cases of B-cell malignant lymphomas of the mixed cell type can be separated from other B-cell lymphomas on the basis of HLA-DR distribution.
AB - Ten cases of reactive follicular hyperplasia and 31 cases of B-cell non-Hodgkin's malignant lymphoma were studied using multiparameter flow cytometry. A bimodal distribution for HLA-DR expression, but not for surface immunoglobulin or B cell-specific antigens CD19 and CD20, was observed commonly in mixed cell type and infrequently in non-mixed cell type B-cell malignant lymphomas. On the basis of HLA-DR distribution alone, 31 case of B-cell malignant lymphomas of low, intermediate, and high grades could be separated into mixed and non-mixed cell types, with only two misclassifications (P=0.0001). Exceptionally, one case of malignant lymphoma, follicular and diffuse, mixed-cell type had a unimodal HLA-DR distribution, and one case of malignant lymphoma, diffuse, large noncleaved cell type had a bimodal HLA-DR distribution. In all cases of malignant lymphoma, follicular, mixed-cell type studied, low HLA-DR was correlated with small cells, and high HLA-DR was correlated with large cells. In contrast, HLA-DR expression and cell size were not as directly correlated in cases of malignant lymphoma, diffuse, mixed-cell type. These observations suggest that most, but not all, cases of B-cell malignant lymphomas of the mixed cell type can be separated from other B-cell lymphomas on the basis of HLA-DR distribution.
KW - B cell
KW - flow cytometry
KW - HLA-DR
KW - major histocompatibility complex class II
KW - malignant lymphoma
UR - http://www.scopus.com/inward/record.url?scp=0025678357&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025678357&partnerID=8YFLogxK
U2 - 10.1016/S0046-8177(06)80042-0
DO - 10.1016/S0046-8177(06)80042-0
M3 - Article
VL - 21
SP - 1275
EP - 1282
JO - Human Pathology
JF - Human Pathology
SN - 0046-8177
IS - 12
ER -