Neuronal cells in culture respond to neuronal growth factors by secondary cellular pathways similar to those described for activation of lymphocytes and macrophages. In HIV-1-infected T lymphocytes and in macrophages, these pathways were shown to converge on nuclear factors that bind and stimulate the HIV-1 LTR and lead to enhancement of HIV-1 expression. In the current study we have investigated whether the same mechanisms also enhance HIV-1 production by neural cells. We have demonstrated that HIV/N1T replication in HCN-1A cells, a human cortical neuronal cell line, is enhanced threefold by nerve growth factor (NGF) and by fibroblast growth factor (FGF), but not by epidermal growth (EGF) factor, or phorbol ester. HCN-1A cells also responded to HIV/N1T infection with pronounced morphological changes, indicative of a differentiation-like process. The cells diminished in size and small neurites were observed.
ASJC Scopus subject areas
- Infectious Diseases
HIV Type 1 Infection of Human Cortical Neuronal Cells : Enhancement by Select Neuronal Growth Factors. / Mizrachi, Yaffa; Rodriguez, Isaac; Sweetnam, Paul M.; Rubinstein, Arye; Volsky, David J.In: AIDS Research and Human Retroviruses, Vol. 10, No. 12, 12.1994, p. 1593-1596.
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