HIV type 1 infection of human cortical neuronal cells: Enhancement by select neuronal growth factors

Y. Mizrachi, I. Rodriguez, P. M. Sweetnam, Arye Rubinstein, D. J. Volsky

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Neuronal cells in culture respond to neuronal growth factors by secondary cellular pathways similar to those described for activation of lymphocytes and macrophages. In HIV-1-infected T lymphocytes and in macrophages, these pathways were shown to converge on nuclear factors that bind and stimulate the HIV-1 LTR and lead lo enhancement of HIV-1 expression. In the current study we have investigated whether the same mechanisms also enhance HIV-1 production by neural cells. We have demonstrated that HIV/N1T replication in HCN-1A cells, a human cortical neuronal cell line, is enhanced threefold by nerve growth factor (NGF) and by fibroblast growth factor (FGF), but not by epidermal growth (EGF) factor, or phorbol ester. HCN-1A cells also responded to HIV/NIT infection with pronounced morphological changes, indicative of a differentiation-like process. The cells diminished in size and small neurites were observed.

Original languageEnglish (US)
Pages (from-to)1593-1596
Number of pages4
JournalAIDS Research and Human Retroviruses
Volume10
Issue number12
StatePublished - 1994

Fingerprint

HIV Infections
HIV-1
Intercellular Signaling Peptides and Proteins
Epidermal Growth Factor
HIV Long Terminal Repeat
Fibroblast Growth Factors
Macrophage Activation
Phorbol Esters
Nerve Growth Factor
Neurites
Lymphocyte Activation
Cell Culture Techniques
Macrophages
HIV
T-Lymphocytes
Cell Line

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

HIV type 1 infection of human cortical neuronal cells : Enhancement by select neuronal growth factors. / Mizrachi, Y.; Rodriguez, I.; Sweetnam, P. M.; Rubinstein, Arye; Volsky, D. J.

In: AIDS Research and Human Retroviruses, Vol. 10, No. 12, 1994, p. 1593-1596.

Research output: Contribution to journalArticle

Mizrachi, Y. ; Rodriguez, I. ; Sweetnam, P. M. ; Rubinstein, Arye ; Volsky, D. J. / HIV type 1 infection of human cortical neuronal cells : Enhancement by select neuronal growth factors. In: AIDS Research and Human Retroviruses. 1994 ; Vol. 10, No. 12. pp. 1593-1596.
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