HIV Type 1 Infection of Human Cortical Neuronal Cells: Enhancement by Select Neuronal Growth Factors

Yaffa Mizrachi, Isaac Rodriguez, Paul M. Sweetnam, Arye Rubinstein, David J. Volsky

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Neuronal cells in culture respond to neuronal growth factors by secondary cellular pathways similar to those described for activation of lymphocytes and macrophages. In HIV-1-infected T lymphocytes and in macrophages, these pathways were shown to converge on nuclear factors that bind and stimulate the HIV-1 LTR and lead to enhancement of HIV-1 expression. In the current study we have investigated whether the same mechanisms also enhance HIV-1 production by neural cells. We have demonstrated that HIV/N1T replication in HCN-1A cells, a human cortical neuronal cell line, is enhanced threefold by nerve growth factor (NGF) and by fibroblast growth factor (FGF), but not by epidermal growth (EGF) factor, or phorbol ester. HCN-1A cells also responded to HIV/N1T infection with pronounced morphological changes, indicative of a differentiation-like process. The cells diminished in size and small neurites were observed.

Original languageEnglish (US)
Pages (from-to)1593-1596
Number of pages4
JournalAIDS Research and Human Retroviruses
Volume10
Issue number12
DOIs
StatePublished - Dec 1994

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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