HIV-1 expression induces tubular cell G2/M arrest and apoptosis

Himanshu Vashistha, Mohammad Husain, Dileep Kumar, Anju Yadav, Shitij Arora, Pravin C. Singhal

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Human renal biopsy studies suggest the presence of HIV-1 and associated signs of injury in renal tubular epithelial cells. Because renal epithelial cells lack conventional HIV-1 receptors, the modus operandi of HIV-1 in the induction of tubular cell injury remains a mystery. In the present study, we evaluated the role of HIV-1 gene expression in human proximal tubular cell apoptosis and cell cycle progression. HIV-1- or vector-transduced cells were assayed for cellular injury and cell cycle defect. HIV-1-transduced cells showed the progressive loss of viability in a time-dependent manner. Similarly, HIV-1-transduced cells showed greater apoptosis when compared with vector-transduced cells. A higher number of HIV-1 expressing cells showed cell cycle arrest at G2/M phase and enhanced tubular cell expression of phospho-p53(ser15), phospho-cdc-2(Tyr 15), and phospho-chk-2 (Thr 68). These findings suggest that in addition to the activation of apoptotic pathway, HIV-1-induced G2/M arrest may also contribute to tubular cell injury.

Original languageEnglish (US)
Pages (from-to)655-664
Number of pages10
JournalRenal Failure
Volume30
Issue number6
DOIs
StatePublished - Jul 1 2008
Externally publishedYes

Keywords

  • Apoptosis
  • Cell cycle
  • HIV-1
  • Tubular cells

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Nephrology

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    Vashistha, H., Husain, M., Kumar, D., Yadav, A., Arora, S., & Singhal, P. C. (2008). HIV-1 expression induces tubular cell G2/M arrest and apoptosis. Renal Failure, 30(6), 655-664. https://doi.org/10.1080/08860220802134672