HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus

Viacheslav Malikov, Eveline Santos Da Silva, Vladimir Jovasevic, Geoffrey Bennett, Daniel A. De Souza Aranha Vieira, Bianca Schulte, Felipe Diaz-Griffero, Derek Walsh, Mojgan H. Naghavi

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Intracellular transport of cargos, including many viruses, involves directed movement on microtubules mediated by motor proteins. Although a number of viruses bind motors of opposing directionality, how they associate with and control these motors to accomplish directed movement remains poorly understood. Here we show that human immunodeficiency virus type 1 (HIV-1) associates with the kinesin-1 adaptor protein, Fasiculation and Elongation Factor zeta 1 (FEZ1). RNAi-mediated FEZ1 depletion blocks early infection, with virus particles exhibiting bi-directional motility but no net movement to the nucleus. Furthermore, both dynein and kinesin-1 motors are required for HIV-1 trafficking to the nucleus. Finally, the ability of exogenously expressed FEZ1 to promote early HIV-1 infection requires binding to kinesin-1. Our findings demonstrate that opposing motors both contribute to early HIV-1 movement and identify the kinesin-1 adaptor, FEZ1 as a capsid-associated host regulator of this process usurped by HIV-1 to accomplish net inward movement towards the nucleus.

Original languageEnglish (US)
Article number6660
JournalNature Communications
Volume6
DOIs
StatePublished - 2015

Fingerprint

Peptide Elongation Factor 1
Peptide Elongation Factors
Kinesin
human immunodeficiency virus
Capsid
Viruses
elongation
HIV-1
viruses
nuclei
infectious diseases
proteins
Dyneins
locomotion
regulators
Virus Diseases
RNA Interference
Microtubules
Virion
depletion

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Malikov, V., Da Silva, E. S., Jovasevic, V., Bennett, G., De Souza Aranha Vieira, D. A., Schulte, B., ... Naghavi, M. H. (2015). HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus. Nature Communications, 6, [6660]. https://doi.org/10.1038/ncomms7660

HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus. / Malikov, Viacheslav; Da Silva, Eveline Santos; Jovasevic, Vladimir; Bennett, Geoffrey; De Souza Aranha Vieira, Daniel A.; Schulte, Bianca; Diaz-Griffero, Felipe; Walsh, Derek; Naghavi, Mojgan H.

In: Nature Communications, Vol. 6, 6660, 2015.

Research output: Contribution to journalArticle

Malikov, V, Da Silva, ES, Jovasevic, V, Bennett, G, De Souza Aranha Vieira, DA, Schulte, B, Diaz-Griffero, F, Walsh, D & Naghavi, MH 2015, 'HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus', Nature Communications, vol. 6, 6660. https://doi.org/10.1038/ncomms7660
Malikov V, Da Silva ES, Jovasevic V, Bennett G, De Souza Aranha Vieira DA, Schulte B et al. HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus. Nature Communications. 2015;6. 6660. https://doi.org/10.1038/ncomms7660
Malikov, Viacheslav ; Da Silva, Eveline Santos ; Jovasevic, Vladimir ; Bennett, Geoffrey ; De Souza Aranha Vieira, Daniel A. ; Schulte, Bianca ; Diaz-Griffero, Felipe ; Walsh, Derek ; Naghavi, Mojgan H. / HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus. In: Nature Communications. 2015 ; Vol. 6.
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