Hits and misses from gene expression ratio measurements in cDNA microarray studies

Dumitru A. Iacobas, Aldo B. Massimi, Marcia Urban, Sanda Iacobas, David C. Spray

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

cDNA microarray users face many challenges to obtain accurate results, including complex technical errors, natural variability of biological systems, imperfect reproducibility of reference standards, and difficulties in acquisition and processing of large amounts of data. Therefore, investigators should be aware of potential sources of variability and account for them in the experimental design and execution. This work reports our experience in identifying factors that alter the reliability of the results and in diminishing effects of these factors. We have studied the hybridization reproducibility in cDNA microarray chips, both as absolute values and expression ratios, and the nature and impact of several technical, acquisition, and processing errors. A new experimental strategy is proposed and mathematical algorithms developed that efficiently correct the errors and thereby increase the information obtainable through microarray studies. These algorithms reduced the variability not associated with biological system to less than a quarter of its initial value and have substantially enhanced reliability in experiments on brain and cultured neuroblastoma cells.

Original languageEnglish (US)
Pages (from-to)143-157
Number of pages15
JournalJournal of Biomolecular Techniques
Volume13
Issue number3
StatePublished - Sep 2002

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Oligonucleotide Array Sequence Analysis
Gene Expression
Neuroblastoma
Reproducibility of Results
Cultured Cells
Research Design
Research Personnel
Brain

Keywords

  • cDNA microarray
  • Error correction
  • Expression ratio
  • Expression variability
  • Hybridization error

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Hits and misses from gene expression ratio measurements in cDNA microarray studies. / Iacobas, Dumitru A.; Massimi, Aldo B.; Urban, Marcia; Iacobas, Sanda; Spray, David C.

In: Journal of Biomolecular Techniques, Vol. 13, No. 3, 09.2002, p. 143-157.

Research output: Contribution to journalArticle

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