Histone H3K23-specific acetylation by MORF is coupled to H3K14 acylation

Brianna J. Klein, Suk Min Jang, Catherine Lachance, Wenyi Mi, Jie Lyu, Shun Sakuraba, Krzysztof Krajewski, Wesley W. Wang, Simone Sidoli, Jiuyang Liu, Yi Zhang, Xiaolu Wang, Becka M. Warfield, Andrew J. Kueh, Anne K. Voss, Tim Thomas, Benjamin A. Garcia, Wenshe R. Liu, Brian D. Strahl, Hidetoshi KonoWei Li, Xiaobing Shi, Jacques Côté, Tatiana G. Kutateladze

Research output: Contribution to journalArticle

Abstract

Acetylation of histone H3K23 has emerged as an essential posttranslational modification associated with cancer and learning and memory impairment, yet our understanding of this epigenetic mark remains insufficient. Here, we identify the native MORF complex as a histone H3K23-specific acetyltransferase and elucidate its mechanism of action. The acetyltransferase function of the catalytic MORF subunit is positively regulated by the DPF domain of MORF (MORFDPF). The crystal structure of MORFDPF in complex with crotonylated H3K14 peptide provides mechanistic insight into selectivity of this epigenetic reader and its ability to recognize both histone and DNA. ChIP data reveal the role of MORFDPF in MORF-dependent H3K23 acetylation of target genes. Mass spectrometry, biochemical and genomic analyses show co-existence of the H3K23ac and H3K14ac modifications in vitro and co-occupancy of the MORF complex, H3K23ac, and H3K14ac at specific loci in vivo. Our findings suggest a model in which interaction of MORFDPF with acylated H3K14 promotes acetylation of H3K23 by the native MORF complex to activate transcription.

Original languageEnglish (US)
Article number4724
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019

Fingerprint

acetylation
acylation
Acetylation
Acylation
Histones
Acetyltransferases
Epigenomics
Aptitude
impairment
readers
Transcription
Post Translational Protein Processing
loci
genes
learning
peptides
Mass spectrometry
Mass Spectrometry
Catalytic Domain
mass spectroscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Klein, B. J., Jang, S. M., Lachance, C., Mi, W., Lyu, J., Sakuraba, S., ... Kutateladze, T. G. (2019). Histone H3K23-specific acetylation by MORF is coupled to H3K14 acylation. Nature communications, 10(1), [4724]. https://doi.org/10.1038/s41467-019-12551-5

Histone H3K23-specific acetylation by MORF is coupled to H3K14 acylation. / Klein, Brianna J.; Jang, Suk Min; Lachance, Catherine; Mi, Wenyi; Lyu, Jie; Sakuraba, Shun; Krajewski, Krzysztof; Wang, Wesley W.; Sidoli, Simone; Liu, Jiuyang; Zhang, Yi; Wang, Xiaolu; Warfield, Becka M.; Kueh, Andrew J.; Voss, Anne K.; Thomas, Tim; Garcia, Benjamin A.; Liu, Wenshe R.; Strahl, Brian D.; Kono, Hidetoshi; Li, Wei; Shi, Xiaobing; Côté, Jacques; Kutateladze, Tatiana G.

In: Nature communications, Vol. 10, No. 1, 4724, 01.12.2019.

Research output: Contribution to journalArticle

Klein, BJ, Jang, SM, Lachance, C, Mi, W, Lyu, J, Sakuraba, S, Krajewski, K, Wang, WW, Sidoli, S, Liu, J, Zhang, Y, Wang, X, Warfield, BM, Kueh, AJ, Voss, AK, Thomas, T, Garcia, BA, Liu, WR, Strahl, BD, Kono, H, Li, W, Shi, X, Côté, J & Kutateladze, TG 2019, 'Histone H3K23-specific acetylation by MORF is coupled to H3K14 acylation', Nature communications, vol. 10, no. 1, 4724. https://doi.org/10.1038/s41467-019-12551-5
Klein BJ, Jang SM, Lachance C, Mi W, Lyu J, Sakuraba S et al. Histone H3K23-specific acetylation by MORF is coupled to H3K14 acylation. Nature communications. 2019 Dec 1;10(1). 4724. https://doi.org/10.1038/s41467-019-12551-5
Klein, Brianna J. ; Jang, Suk Min ; Lachance, Catherine ; Mi, Wenyi ; Lyu, Jie ; Sakuraba, Shun ; Krajewski, Krzysztof ; Wang, Wesley W. ; Sidoli, Simone ; Liu, Jiuyang ; Zhang, Yi ; Wang, Xiaolu ; Warfield, Becka M. ; Kueh, Andrew J. ; Voss, Anne K. ; Thomas, Tim ; Garcia, Benjamin A. ; Liu, Wenshe R. ; Strahl, Brian D. ; Kono, Hidetoshi ; Li, Wei ; Shi, Xiaobing ; Côté, Jacques ; Kutateladze, Tatiana G. / Histone H3K23-specific acetylation by MORF is coupled to H3K14 acylation. In: Nature communications. 2019 ; Vol. 10, No. 1.
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AU - Thomas, Tim

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AU - Strahl, Brian D.

AU - Kono, Hidetoshi

AU - Li, Wei

AU - Shi, Xiaobing

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