Histone H1 depletion in mammals alters global chromatin structure but causes specific changes in gene regulation

Yuhong Fan, Tatiana Nikitina, Jie Zhao, Tomara J. Fleury, Riddhi Bhattacharyya, Eric E. Bouhassira, Arnold Stein, Christopher L. Woodcock, Arthur I. Skoultchi

Research output: Contribution to journalArticle

346 Citations (Scopus)

Abstract

Linker histone H1 plays an important role in chromatin folding in vitro. To study the role of H1 in vivo, mouse embryonic stem cells null for three H1 genes were derived and were found to have 50% of the normal level of H1. H1 depletion caused dramatic chromatin structure changes, including decreased global nucleosome spacing, reduced local chromatin compaction, and decreases in certain core histone modifications. Surprisingly, however, microarray analysis revealed that expression of only a small number of genes is affected. Many of the affected genes are imprinted or are on the X chromosome and are therefore normally regulated by DNA methylation. Although global DNA methylation is not changed, methylation of specific CpGs within the regulatory regions of some of the H1 regulated genes is reduced. These results indicate that linker histones can participate in epigenetic regulation of gene expression by contributing to the maintenance or establishment of specific DNA methylation patterns.

Original languageEnglish (US)
Pages (from-to)1199-1212
Number of pages14
JournalCell
Volume123
Issue number7
DOIs
StatePublished - Dec 29 2005

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Mammals
Gene expression
Histones
Chromatin
DNA Methylation
Genes
Histone Code
Methylation
Nucleosomes
Nucleic Acid Regulatory Sequences
Gene Expression Regulation
X Chromosome
Microarray Analysis
Microarrays
Chromosomes
Stem cells
Epigenomics
Compaction
Maintenance

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Histone H1 depletion in mammals alters global chromatin structure but causes specific changes in gene regulation. / Fan, Yuhong; Nikitina, Tatiana; Zhao, Jie; Fleury, Tomara J.; Bhattacharyya, Riddhi; Bouhassira, Eric E.; Stein, Arnold; Woodcock, Christopher L.; Skoultchi, Arthur I.

In: Cell, Vol. 123, No. 7, 29.12.2005, p. 1199-1212.

Research output: Contribution to journalArticle

Fan, Y, Nikitina, T, Zhao, J, Fleury, TJ, Bhattacharyya, R, Bouhassira, EE, Stein, A, Woodcock, CL & Skoultchi, AI 2005, 'Histone H1 depletion in mammals alters global chromatin structure but causes specific changes in gene regulation', Cell, vol. 123, no. 7, pp. 1199-1212. https://doi.org/10.1016/j.cell.2005.10.028
Fan, Yuhong ; Nikitina, Tatiana ; Zhao, Jie ; Fleury, Tomara J. ; Bhattacharyya, Riddhi ; Bouhassira, Eric E. ; Stein, Arnold ; Woodcock, Christopher L. ; Skoultchi, Arthur I. / Histone H1 depletion in mammals alters global chromatin structure but causes specific changes in gene regulation. In: Cell. 2005 ; Vol. 123, No. 7. pp. 1199-1212.
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