Higher order structure is present in the yeast nucleus

autoantibody probes demonstrate that the nucleolus lies opposite the spindle pole body

Charles H. Yang, Eric J. Lambie, John Hardin, Joe Craft, Michael Snyder

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

A panel of sera from 892 autoimmune patients was screened by indirect immunofluorescence on mammalian cells. Seventy-three sera were identified that recognize the nucleolus. Three of these sera appear to stain the nucleolus in yeast, suggesting that they recognize highly conserved antigens. These three sera also immunoprecipitate mammalian U3 snRNA-containing particles, which reside in the nucleolus and have been implicated in rRNA processing. Double immunofluorescence experiments with anti-nucleolus and anti-tubulin antibodies revealed a novel form of non-random nuclear organization in yeast. The spindle pole body and the nucleolus - both of which are associated with the nuclear envelope - preferentially localize at opposite ends of the nucleus. Organization of these and other components into specific regions of the nucleus may be important for optimizing their proper function.

Original languageEnglish (US)
Pages (from-to)123-128
Number of pages6
JournalChromosoma
Volume98
Issue number2
DOIs
StatePublished - Aug 1989
Externally publishedYes

Fingerprint

Spindle Pole Bodies
Autoantibodies
Yeasts
Serum
Nuclear Envelope
Tubulin
Indirect Fluorescent Antibody Technique
Fluorescent Antibody Technique
Anti-Idiotypic Antibodies
Coloring Agents
Antigens

ASJC Scopus subject areas

  • Genetics

Cite this

Higher order structure is present in the yeast nucleus : autoantibody probes demonstrate that the nucleolus lies opposite the spindle pole body. / Yang, Charles H.; Lambie, Eric J.; Hardin, John; Craft, Joe; Snyder, Michael.

In: Chromosoma, Vol. 98, No. 2, 08.1989, p. 123-128.

Research output: Contribution to journalArticle

Yang, Charles H. ; Lambie, Eric J. ; Hardin, John ; Craft, Joe ; Snyder, Michael. / Higher order structure is present in the yeast nucleus : autoantibody probes demonstrate that the nucleolus lies opposite the spindle pole body. In: Chromosoma. 1989 ; Vol. 98, No. 2. pp. 123-128.
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