High sensitivity MEMS biosensor for monitoring cell attachment

Fei Liu, Fang Li, David C. Spray, Anis Nurashikin Nordin, Ioana Voiculescu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper presents the fabrication and testing of a novel microelectromechanical (MEMS) biosensor based on live cells. The biosensor combines two biosensing techniques; resonant frequency measurements and electric cellsubstrate impedance sensing (ECIS) on a single device. The sensor is based on the innovative placement of the working microelectrode for ECIS technique as the upper electrode of a quartz crystal microbalance (QCM) resonator. This hybrid biosensor was tested with bovine aortic endothelial cells with different seeding densities. The cell attachment and spreading was monitored with both sensors; the QCM and the ECIS technique. After the cells form a monolayer the values of the impedance and resonant frequency measurements are constant. The optimal cell seeding density with minimal time required to attach and form a monolayer was observed to be 1.5×104 cells/cm2. This biosensor monitors the cells attachment and viability and could be used for screening toxicants in drinking water.

Original languageEnglish (US)
Title of host publicationASME 2012 International Mechanical Engineering Congress and Exposition, IMECE 2012
Pages67-71
Number of pages5
EditionPARTS A AND B
DOIs
StatePublished - Dec 1 2012
EventASME 2012 International Mechanical Engineering Congress and Exposition, IMECE 2012 - Houston, TX, United States
Duration: Nov 9 2012Nov 15 2012

Publication series

NameASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
NumberPARTS A AND B
Volume9

Other

OtherASME 2012 International Mechanical Engineering Congress and Exposition, IMECE 2012
CountryUnited States
CityHouston, TX
Period11/9/1211/15/12

ASJC Scopus subject areas

  • Mechanical Engineering

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  • Cite this

    Liu, F., Li, F., Spray, D. C., Nordin, A. N., & Voiculescu, I. (2012). High sensitivity MEMS biosensor for monitoring cell attachment. In ASME 2012 International Mechanical Engineering Congress and Exposition, IMECE 2012 (PARTS A AND B ed., pp. 67-71). (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE); Vol. 9, No. PARTS A AND B). https://doi.org/10.1115/IMECE2012-85613