High sensitivity immunolocalization of double and single-stranded DNA by a monoclonal antibody.

U. Scheer, K. Messner, R. Hazan, I. Raska, P. Hansmann, H. Falk, E. Spiess, W. W. Franke

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

A monoclonal antibody (AK 30-10) is described which specifically reacts with DNA both in double and single-stranded forms but not with other molecules and structures, including deoxyribonucleotides and RNAs. When used in immunocytochemical experiments on tissue sections and permeabilized cultured cells, this antibody detects DNA-containing structures, even when the DNA is present in very small amounts. Examples of high resolution detection include the DNA present in amplified extrachromosomal nucleoli, chromomeres of lampbrush chromosomes, mitochondria, chloroplasts and mycoplasmal particles. In immunoelectron microscopy using the immunogold technique, the DNA was localized in distinct substructures such as the "fibrillar centers" of nucleoli and certain stromal centers in chloroplasts. The antibody also reacts with DNA of chromatin of living cells, as shown by microinjection into cultured mitotic cells and into nuclei of amphibian oocytes. The potential value and the limitations of immunocytochemical DNA detection are discussed.

Original languageEnglish (US)
Pages (from-to)358-371
Number of pages14
JournalEuropean Journal of Cell Biology
Volume43
Issue number3
StatePublished - Jun 1 1987
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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    Scheer, U., Messner, K., Hazan, R., Raska, I., Hansmann, P., Falk, H., Spiess, E., & Franke, W. W. (1987). High sensitivity immunolocalization of double and single-stranded DNA by a monoclonal antibody. European Journal of Cell Biology, 43(3), 358-371.