High risk first degree relatives of type 1 diabetics: An association with increases in CXCR3+ T memory cells reflecting an enhanced activity of Th1 autoimmune response

Tanja Milicic, Aleksandra Jotic, Ivanka Markovic, Katarina Lalic, Veljko Jeremic, Ljiljana Lukic, Natasa Rajkovic, Dušan Popadic, Marija Macesic, Jelena P. Seferovic, Sandra Aleksic, Jelena Stanarcic, Milorad Civcic, Nebojsa M. Lalic

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We analyzed the level of (a) CXCR3+ (Th1) and CCR4+ (Th2) T memory cells (b) interferon-γ inducible chemokine (IP-10)(Th1) and thymus and activation-regulated chemokine (TARC)(Th2), in 51 first degree relatives (FDRs) of type 1 diabetics (T1D) (17 high risk FDRs (GADA+, IA-2+) and 34 low risk FDRs (GADA-, IA-2-)), 24 recent-onset T1D (R-T1D), and 18 healthy subjects. T memory subsets were analyzed by using four-color immunofluorescence staining and flowcytometry. IP-10 and TARC were determined by ELISA. High risk FDRs showed higher levels of CXCR3+ and lower level of CCR4+ T memory cells compared to low risk FDRs (64.98 ± 5.19 versus 42.13 ± 11.11; 29.46 ± 2.83 versus 41.90 ± 8.58%, resp., P < 0.001). Simultaneously, both IP-10 and TARC levels were increased in high risk versus low risk FDRs (160.12 ± 73.40 versus 105.39 ± 71.30; 438.83 ± 120.62 versus 312.04 ± 151.14 pg/mL, P < 0.05). Binary logistic regression analysis identified the level of CXCR3+ T memory cells as predictors for high risk FDRs, together with high levels of IP-10. The results imply that, in FDRs, the risk for T1D might be strongly influenced by enhanced activity of Th1 and diminished activity of Th2 autoimmune response.

Original languageEnglish (US)
Article number589360
JournalInternational Journal of Endocrinology
Volume2014
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Autoimmunity
Chemokine CCL17
Chemokines
Interferons
Fluorescent Antibody Technique
Healthy Volunteers
Color
Logistic Models
Enzyme-Linked Immunosorbent Assay
Regression Analysis
Staining and Labeling

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems

Cite this

High risk first degree relatives of type 1 diabetics : An association with increases in CXCR3+ T memory cells reflecting an enhanced activity of Th1 autoimmune response. / Milicic, Tanja; Jotic, Aleksandra; Markovic, Ivanka; Lalic, Katarina; Jeremic, Veljko; Lukic, Ljiljana; Rajkovic, Natasa; Popadic, Dušan; Macesic, Marija; Seferovic, Jelena P.; Aleksic, Sandra; Stanarcic, Jelena; Civcic, Milorad; Lalic, Nebojsa M.

In: International Journal of Endocrinology, Vol. 2014, 589360, 2014.

Research output: Contribution to journalArticle

Milicic, T, Jotic, A, Markovic, I, Lalic, K, Jeremic, V, Lukic, L, Rajkovic, N, Popadic, D, Macesic, M, Seferovic, JP, Aleksic, S, Stanarcic, J, Civcic, M & Lalic, NM 2014, 'High risk first degree relatives of type 1 diabetics: An association with increases in CXCR3+ T memory cells reflecting an enhanced activity of Th1 autoimmune response', International Journal of Endocrinology, vol. 2014, 589360. https://doi.org/10.1155/2014/589360
Milicic, Tanja ; Jotic, Aleksandra ; Markovic, Ivanka ; Lalic, Katarina ; Jeremic, Veljko ; Lukic, Ljiljana ; Rajkovic, Natasa ; Popadic, Dušan ; Macesic, Marija ; Seferovic, Jelena P. ; Aleksic, Sandra ; Stanarcic, Jelena ; Civcic, Milorad ; Lalic, Nebojsa M. / High risk first degree relatives of type 1 diabetics : An association with increases in CXCR3+ T memory cells reflecting an enhanced activity of Th1 autoimmune response. In: International Journal of Endocrinology. 2014 ; Vol. 2014.
@article{bf33d025ae664592bf8977d6dece441e,
title = "High risk first degree relatives of type 1 diabetics: An association with increases in CXCR3+ T memory cells reflecting an enhanced activity of Th1 autoimmune response",
abstract = "We analyzed the level of (a) CXCR3+ (Th1) and CCR4+ (Th2) T memory cells (b) interferon-γ inducible chemokine (IP-10)(Th1) and thymus and activation-regulated chemokine (TARC)(Th2), in 51 first degree relatives (FDRs) of type 1 diabetics (T1D) (17 high risk FDRs (GADA+, IA-2+) and 34 low risk FDRs (GADA-, IA-2-)), 24 recent-onset T1D (R-T1D), and 18 healthy subjects. T memory subsets were analyzed by using four-color immunofluorescence staining and flowcytometry. IP-10 and TARC were determined by ELISA. High risk FDRs showed higher levels of CXCR3+ and lower level of CCR4+ T memory cells compared to low risk FDRs (64.98 ± 5.19 versus 42.13 ± 11.11; 29.46 ± 2.83 versus 41.90 ± 8.58{\%}, resp., P < 0.001). Simultaneously, both IP-10 and TARC levels were increased in high risk versus low risk FDRs (160.12 ± 73.40 versus 105.39 ± 71.30; 438.83 ± 120.62 versus 312.04 ± 151.14 pg/mL, P < 0.05). Binary logistic regression analysis identified the level of CXCR3+ T memory cells as predictors for high risk FDRs, together with high levels of IP-10. The results imply that, in FDRs, the risk for T1D might be strongly influenced by enhanced activity of Th1 and diminished activity of Th2 autoimmune response.",
author = "Tanja Milicic and Aleksandra Jotic and Ivanka Markovic and Katarina Lalic and Veljko Jeremic and Ljiljana Lukic and Natasa Rajkovic and Dušan Popadic and Marija Macesic and Seferovic, {Jelena P.} and Sandra Aleksic and Jelena Stanarcic and Milorad Civcic and Lalic, {Nebojsa M.}",
year = "2014",
doi = "10.1155/2014/589360",
language = "English (US)",
volume = "2014",
journal = "International Journal of Endocrinology",
issn = "1687-8337",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - High risk first degree relatives of type 1 diabetics

T2 - An association with increases in CXCR3+ T memory cells reflecting an enhanced activity of Th1 autoimmune response

AU - Milicic, Tanja

AU - Jotic, Aleksandra

AU - Markovic, Ivanka

AU - Lalic, Katarina

AU - Jeremic, Veljko

AU - Lukic, Ljiljana

AU - Rajkovic, Natasa

AU - Popadic, Dušan

AU - Macesic, Marija

AU - Seferovic, Jelena P.

AU - Aleksic, Sandra

AU - Stanarcic, Jelena

AU - Civcic, Milorad

AU - Lalic, Nebojsa M.

PY - 2014

Y1 - 2014

N2 - We analyzed the level of (a) CXCR3+ (Th1) and CCR4+ (Th2) T memory cells (b) interferon-γ inducible chemokine (IP-10)(Th1) and thymus and activation-regulated chemokine (TARC)(Th2), in 51 first degree relatives (FDRs) of type 1 diabetics (T1D) (17 high risk FDRs (GADA+, IA-2+) and 34 low risk FDRs (GADA-, IA-2-)), 24 recent-onset T1D (R-T1D), and 18 healthy subjects. T memory subsets were analyzed by using four-color immunofluorescence staining and flowcytometry. IP-10 and TARC were determined by ELISA. High risk FDRs showed higher levels of CXCR3+ and lower level of CCR4+ T memory cells compared to low risk FDRs (64.98 ± 5.19 versus 42.13 ± 11.11; 29.46 ± 2.83 versus 41.90 ± 8.58%, resp., P < 0.001). Simultaneously, both IP-10 and TARC levels were increased in high risk versus low risk FDRs (160.12 ± 73.40 versus 105.39 ± 71.30; 438.83 ± 120.62 versus 312.04 ± 151.14 pg/mL, P < 0.05). Binary logistic regression analysis identified the level of CXCR3+ T memory cells as predictors for high risk FDRs, together with high levels of IP-10. The results imply that, in FDRs, the risk for T1D might be strongly influenced by enhanced activity of Th1 and diminished activity of Th2 autoimmune response.

AB - We analyzed the level of (a) CXCR3+ (Th1) and CCR4+ (Th2) T memory cells (b) interferon-γ inducible chemokine (IP-10)(Th1) and thymus and activation-regulated chemokine (TARC)(Th2), in 51 first degree relatives (FDRs) of type 1 diabetics (T1D) (17 high risk FDRs (GADA+, IA-2+) and 34 low risk FDRs (GADA-, IA-2-)), 24 recent-onset T1D (R-T1D), and 18 healthy subjects. T memory subsets were analyzed by using four-color immunofluorescence staining and flowcytometry. IP-10 and TARC were determined by ELISA. High risk FDRs showed higher levels of CXCR3+ and lower level of CCR4+ T memory cells compared to low risk FDRs (64.98 ± 5.19 versus 42.13 ± 11.11; 29.46 ± 2.83 versus 41.90 ± 8.58%, resp., P < 0.001). Simultaneously, both IP-10 and TARC levels were increased in high risk versus low risk FDRs (160.12 ± 73.40 versus 105.39 ± 71.30; 438.83 ± 120.62 versus 312.04 ± 151.14 pg/mL, P < 0.05). Binary logistic regression analysis identified the level of CXCR3+ T memory cells as predictors for high risk FDRs, together with high levels of IP-10. The results imply that, in FDRs, the risk for T1D might be strongly influenced by enhanced activity of Th1 and diminished activity of Th2 autoimmune response.

UR - http://www.scopus.com/inward/record.url?scp=84899553489&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899553489&partnerID=8YFLogxK

U2 - 10.1155/2014/589360

DO - 10.1155/2014/589360

M3 - Article

AN - SCOPUS:84899553489

VL - 2014

JO - International Journal of Endocrinology

JF - International Journal of Endocrinology

SN - 1687-8337

M1 - 589360

ER -