High-resolution proteomic mapping in the vertebrate central nervous system

Close proximity of connexin35 to NMDA glutamate receptor clusters and co-localization of connexin36 with immunoreactivity for zonula occludens protein-1 (ZO-1)

J. E. Rash, Alberto E. Pereda, N. Kamasawa, C. S. Furman, T. Yasumura, K. G V Davidson, F. E. Dudek, C. Olson, X. Li, J. I. Nagy

Research output: Contribution to journalArticle

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Abstract

Combined confocal microscopy and freeze-fracture replica immunogold labeling (FRIL) were used to examine the connexin identity at electrical synapses in goldfish brain and rat retina, and to test for "co- localization" vs. "close proximity" of connexins to other functionally interacting proteins in synapses of goldfish and mouse brain and rat retina. In goldfish brain, confocal microscopy revealed immunofluorescence for connexin35 (Cx35) and NMDA-R1 (NR1) glutamate receptor protein in Mauthner Cell/Club Ending synapses. By FRIL double labeling, NR1 glutamate receptors were found in clusters of intramembrane particles in the postsynaptic membrane extraplasmic leaflets, and these distinctive postsynaptic densities were in close proximity (0.1-0.3 μm) to neuronal gap junctions labeled for Cx35, which is the fish ortholog of connexin36 (Cx36) found at neuronal gap junctions in mammals. Immunogold labeling for Cx36 in adult rat retina revealed abundant gap junctions, including several previously unrecognized morphological types. As in goldfish hindbrain, immunogold double labeling revealed NR1-containing postsynaptic densities localized near Cx36-labeled gap junction in rat inferior olive. Confocal immunofluorescence microscopy revealed widespread co-localization of Cx36 and ZO-1, particularly in the reticular thalamic nucleus and amygdala of mouse brain. By FRIL, ZO-1 immunoreactivity was co-localized with Cx36 at individual gap junction plaques in rat retinal neurons. As cytoplasmic accessory proteins, ZO-1 and possibly related members of the membrane-associated guanylate kinase (MAGUK) family represent scaffolding proteins that may bind to and regulate the activity of many neuronal gap junctions. These data document the power of combining immunofluorescence confocal microscopy with FRIL ultrastructural imaging and immunogold labeling to determine the relative proximities of proteins that are involved in short- vs. intermediate-range molecular interactions in the complex membrane appositions at synapses between neurons.

Original languageEnglish (US)
Pages (from-to)131-151
Number of pages21
JournalJournal of Neurocytology
Volume33
Issue number1 SPEC. ISS.
DOIs
StatePublished - Jan 2004

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Zonula Occludens-1 Protein
Electrical Synapses
Glutamate Receptors
N-Methyl-D-Aspartate Receptors
Goldfish
Proteomics
Vertebrates
Central Nervous System
Confocal Microscopy
Gap Junctions
N-Methylaspartate
Synapses
Post-Synaptic Density
Retina
Connexins
Brain
Proteins
Fluorescence Microscopy
Guanylate Kinases
Retinal Neurons

ASJC Scopus subject areas

  • Neuroscience(all)
  • Anatomy
  • Cell Biology
  • Histology

Cite this

High-resolution proteomic mapping in the vertebrate central nervous system : Close proximity of connexin35 to NMDA glutamate receptor clusters and co-localization of connexin36 with immunoreactivity for zonula occludens protein-1 (ZO-1). / Rash, J. E.; Pereda, Alberto E.; Kamasawa, N.; Furman, C. S.; Yasumura, T.; Davidson, K. G V; Dudek, F. E.; Olson, C.; Li, X.; Nagy, J. I.

In: Journal of Neurocytology, Vol. 33, No. 1 SPEC. ISS., 01.2004, p. 131-151.

Research output: Contribution to journalArticle

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