High-resolution multi-photon imaging of morphological structures of Caenorhabditis elegans

Gabriele M. Bixel, Stephanie J B Fretham, Michael Aschner

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this protocol, we combine two-photon excitation fluorescence with nonlinear optical measurements to reconstruct the three-dimensional architecture of the pharyngeal region and the muscular system of the anterior and mid-body region of Caenorhabditis elegans (C. elegans). Femto-second laser pulses excite second-harmonic generation (SHG) and third-harmonic generation (THG) signals, which show detailed structural information regarding the organization of myofibrils that are arranged around the central pharynx region. The combination of two-photon excitation with SHG and THG imaging is a very powerful tool to study cell morphology, microarchitecture, and tissue arrangement in C. elegans.

Original languageEnglish (US)
Pages (from-to)11.19.1-11.19.11
JournalCurrent Protocols in Toxicology
Volume2015
DOIs
StatePublished - 2015

Fingerprint

Caenorhabditis elegans
Harmonic generation
Photons
Imaging techniques
Body Regions
Myofibrils
Pharynx
Lasers
Fluorescence
Laser pulses
Tissue

Keywords

  • C. elegans
  • Label-free imaging
  • Multi-photon microscopy
  • SHG
  • THG

ASJC Scopus subject areas

  • Toxicology

Cite this

High-resolution multi-photon imaging of morphological structures of Caenorhabditis elegans. / Bixel, Gabriele M.; Fretham, Stephanie J B; Aschner, Michael.

In: Current Protocols in Toxicology, Vol. 2015, 2015, p. 11.19.1-11.19.11.

Research output: Contribution to journalArticle

Bixel, Gabriele M. ; Fretham, Stephanie J B ; Aschner, Michael. / High-resolution multi-photon imaging of morphological structures of Caenorhabditis elegans. In: Current Protocols in Toxicology. 2015 ; Vol. 2015. pp. 11.19.1-11.19.11.
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