Abstract
In this protocol, we combine two-photon excitation fluorescence with nonlinear optical measurements to reconstruct the three-dimensional architecture of the pharyngeal region and the muscular system of the anterior and mid-body region of Caenorhabditis elegans (C. elegans). Femto-second laser pulses excite second-harmonic generation (SHG) and third-harmonic generation (THG) signals, which show detailed structural information regarding the organization of myofibrils that are arranged around the central pharynx region. The combination of two-photon excitation with SHG and THG imaging is a very powerful tool to study cell morphology, microarchitecture, and tissue arrangement in C. elegans.
Original language | English (US) |
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Pages (from-to) | 11.19.1-11.19.11 |
Journal | Current protocols in toxicology |
Volume | 2015 |
DOIs | |
State | Published - 2015 |
Keywords
- C. elegans
- Label-free imaging
- Multi-photon microscopy
- SHG
- THG
ASJC Scopus subject areas
- Toxicology