High resolution map of Caenorhabditis elegans gap junction proteins

Zeynep F. Altun, Bojun Chen, Zhao Weng Wang, David H. Hall

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The innexin family of gap junction proteins has 25 members in Caenorhabditis elegans. Here, we describe the first high-resolution expression map of all members through analysis of live worms transformed with green fluorescent protein under the control of entire promoter regions. Our analyses show that innexins have dynamic expression patterns throughout development and are found in virtually all cell types and tissues. Complex tissues, such as the pharynx, intestine, gonad, as well as scaffolding tissues and guidepost cells express a variety of innexins in overlapping or complementary patterns, suggesting they may form heteromeric and heterotypic channels. Innexin expression occurs in several types of cells that are not known to form gap junctions as well as in a pair of migrating cells, suggesting they may have hemichannel function. Therefore, innexins likely play roles in almost all body functions, including embryonic development, cell fate determination, oogenesis, egg laying, pharyngeal pumping, excretion, and locomotion.

Original languageEnglish (US)
Pages (from-to)1936-1950
Number of pages15
JournalDevelopmental Dynamics
Volume238
Issue number8
DOIs
StatePublished - Aug 2008

Fingerprint

Caenorhabditis elegans Proteins
Connexins
Tissue Scaffolds
Oogenesis
Gap Junctions
Caenorhabditis elegans
Gonads
Locomotion
Pharynx
Green Fluorescent Proteins
Genetic Promoter Regions
Intestines
Embryonic Development
Ovum

Keywords

  • C. elegans
  • Electrical coupling
  • Gap junction
  • Innexin
  • Intercellular signaling

ASJC Scopus subject areas

  • Developmental Biology

Cite this

High resolution map of Caenorhabditis elegans gap junction proteins. / Altun, Zeynep F.; Chen, Bojun; Wang, Zhao Weng; Hall, David H.

In: Developmental Dynamics, Vol. 238, No. 8, 08.2008, p. 1936-1950.

Research output: Contribution to journalArticle

Altun, Zeynep F. ; Chen, Bojun ; Wang, Zhao Weng ; Hall, David H. / High resolution map of Caenorhabditis elegans gap junction proteins. In: Developmental Dynamics. 2008 ; Vol. 238, No. 8. pp. 1936-1950.
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