High-resolution live-cell imaging and time-lapse microscopy of invadopodium dynamics and tracking analysis

Research output: Chapter in Book/Report/Conference proceedingChapter

22 Scopus citations

Abstract

Invadopodia are specialized structures of cancer cells which aid in cancer cell invasion and metastasis. Therefore, studying the early steps of invadopodium assembly and its life cycle at the subcellular level by using high spatiotemporal resolution imaging provides an opportunity for understanding the signaling mechanisms involved in this very important process. In this chapter, we describe the design of a custom-built high-resolution fluorescence microscope which makes this challenging imaging possible. We also describe an ImageJ plugin that we have developed for tracking of invadopodia and lifetime analysis.

Original languageEnglish (US)
Title of host publicationAdhesion Protein Protocols
PublisherHumana Press Inc.
Pages343-357
Number of pages15
ISBN (Print)9781627035378
DOIs
StatePublished - Jan 1 2013

Publication series

NameMethods in Molecular Biology
Volume1046
ISSN (Print)1064-3745

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Keywords

  • Autofocus
  • Breast carcinoma cells
  • Gelatin degradation
  • Invadopodia
  • Live-cell imaging
  • Particle tracking
  • Time-lapse microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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