High-resolution live-cell imaging and time-lapse microscopy of invadopodium dynamics and tracking analysis

Research output: Chapter in Book/Report/Conference proceedingChapter

22 Citations (Scopus)

Abstract

Invadopodia are specialized structures of cancer cells which aid in cancer cell invasion and metastasis. Therefore, studying the early steps of invadopodium assembly and its life cycle at the subcellular level by using high spatiotemporal resolution imaging provides an opportunity for understanding the signaling mechanisms involved in this very important process. In this chapter, we describe the design of a custom-built high-resolution fluorescence microscope which makes this challenging imaging possible. We also describe an ImageJ plugin that we have developed for tracking of invadopodia and lifetime analysis.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages343-357
Number of pages15
Volume1046
ISBN (Print)9781627035378
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1046
ISSN (Print)10643745

Fingerprint

Time-Lapse Imaging
Microscopy
Life Cycle Stages
Neoplasms
Fluorescence
Neoplasm Metastasis
Podosomes

Keywords

  • Autofocus
  • Breast carcinoma cells
  • Gelatin degradation
  • Invadopodia
  • Live-cell imaging
  • Particle tracking
  • Time-lapse microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

High-resolution live-cell imaging and time-lapse microscopy of invadopodium dynamics and tracking analysis. / Sharma, Ved P.; Entenberg, David R.; Condeelis, John S.

Methods in Molecular Biology. Vol. 1046 Humana Press Inc., 2013. p. 343-357 (Methods in Molecular Biology; Vol. 1046).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sharma, Ved P. ; Entenberg, David R. ; Condeelis, John S. / High-resolution live-cell imaging and time-lapse microscopy of invadopodium dynamics and tracking analysis. Methods in Molecular Biology. Vol. 1046 Humana Press Inc., 2013. pp. 343-357 (Methods in Molecular Biology).
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