High performance mass spectrometry based proteomics reveals enzyme and signaling pathway regulation in neutrophils during the early stage of surgical trauma

Samina Arshid, Muhammad Tahir, Belchor Fontes, Edna Frasson de Souza Montero, Mariana S. Castro, Simone Sidoli, Peter Roepstorff, Wagner Fontes

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Purpose: In clinical conditions trauma is associated with high mortality and morbidity. Neutrophils play a key role in the development of multiple organ failure after trauma. Experimental design: To have a detailed understanding of the neutrophil activation at primary stages after trauma, neutrophils are isolated from control and surgical trauma rats in this study. Extracted proteins are analyzed using nano liquid chromatography coupled with tandem mass spectrometry. Results: A total of 2924 rat neutrophil proteins are identified in our analysis, of which 393 are found differentially regulated between control and trauma groups. By using functional pathways analysis of the 190 proteins up-regulated in surgical trauma, we found proteins related to transcription initiation and protein biosynthesis. On the other hand, among the 203 proteins down-regulated in surgical trauma we found enrichment for proteins of the immune response, proteasome degradation and actin cytoskeleton. Overall, enzyme prediction analysis revealed that regulated enzymes are directly involved in neutrophil apoptosis, directional migration and chemotaxis. Our observations are then confirmed by in silico protein–protein interaction analysis. Conclusions and clinical relevance: Collectively, our results reveal that neutrophils drastically regulate their biochemical pathways after the early stages of surgical trauma, showing lower activity. This implies higher susceptibility of the trauma patients to infection and bystander tissues damage.

Original languageEnglish (US)
Article number1600001
JournalProteomics - Clinical Applications
Volume11
Issue number1-2
DOIs
StatePublished - Jan 1 2017

Keywords

  • Inflammation
  • Neutrophil
  • Proteome
  • Surgery
  • Trauma

ASJC Scopus subject areas

  • Clinical Biochemistry

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