Abstract
High-performance anion-exchange chromatography of oxidized and reduced forms of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP) on a Pharmacia Mono Q anion-exchange column is reported. Microgram quantities of all four nucleotides can be separated at pH 7.7 in approximately 20 min. For preparative purposes, >7 mg of NADH can be purified in a single injection, and the peak fractions have an A260 of greater than 80 OD units with an A260 A340 ratio of 2.25.
Original language | English (US) |
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Pages (from-to) | 232-234 |
Number of pages | 3 |
Journal | Analytical Biochemistry |
Volume | 142 |
Issue number | 1 |
DOIs | |
State | Published - Oct 1984 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology