High-performance ion-exchange separation of oxidized and reduced nicotinamide adenine dinucleotides

George A. Orr, John S. Blanchard

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

High-performance anion-exchange chromatography of oxidized and reduced forms of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP) on a Pharmacia Mono Q anion-exchange column is reported. Microgram quantities of all four nucleotides can be separated at pH 7.7 in approximately 20 min. For preparative purposes, >7 mg of NADH can be purified in a single injection, and the peak fractions have an A260 of greater than 80 OD units with an A260 A340 ratio of 2.25.

Original languageEnglish (US)
Pages (from-to)232-234
Number of pages3
JournalAnalytical Biochemistry
Volume142
Issue number1
DOIs
Publication statusPublished - Oct 1984

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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