High-performance gel-permeation chromatography of polypeptides in a volatile solvent: Rapid resolution and molecular weight estimations of proteins and peptides on a column of TSK-G3000-PW

Gary D. Swergold; Charles S. Rubin

doi:10.1016/0003-2697(83)90173-2

High-performance gel-permeation chromatography of polypeptides in a volatile solvent: Rapid resolution and molecular weight estimations of proteins and peptides on a column of TSK-G3000-PW

Gary D. Swergold, Charles S. Rubin

Molecular Pharmacology

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

A gel-permeation column of TSK-G3000-PW that was equilibrated and developed with 36 or 45% acetonitrile in 0.1% trifluoroacetic acid fractionated mixtures of peptides with high resolving power. In addition, the elution volumes of 11 standard peptides and proteins were linearly related to the logarithms of their molecular weights in the acetonitrile-trifluoroacetic acid solvent at both low and high flow rates. Since the solvent is volatile and relatively transparent to short-wavelength ultraviolet light this high-performance gel-permeation system offers a rapid and highly sensitive method for the analysis, characterization, and purification of peptides and proteins from complex mixtures.

Original language	English (US)
Pages (from-to)	295-300
Number of pages	6
Journal	Analytical Biochemistry
Volume	131
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(83)90173-2
State	Published - Jun 1983

Keywords

gel-permeation chromatography
peptide molecular weights

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "High-performance gel-permeation chromatography of polypeptides in a volatile solvent: Rapid resolution and molecular weight estimations of proteins and peptides on a column of TSK-G3000-PW",

abstract = "A gel-permeation column of TSK-G3000-PW that was equilibrated and developed with 36 or 45% acetonitrile in 0.1% trifluoroacetic acid fractionated mixtures of peptides with high resolving power. In addition, the elution volumes of 11 standard peptides and proteins were linearly related to the logarithms of their molecular weights in the acetonitrile-trifluoroacetic acid solvent at both low and high flow rates. Since the solvent is volatile and relatively transparent to short-wavelength ultraviolet light this high-performance gel-permeation system offers a rapid and highly sensitive method for the analysis, characterization, and purification of peptides and proteins from complex mixtures.",

keywords = "gel-permeation chromatography, peptide molecular weights",

author = "Swergold, {Gary D.} and Rubin, {Charles S.}",

note = "Funding Information: This study was supported by NIH Grants AM 2 1248 and GM 22792. G.D.S. is supported by Medical Scientist Training Grant 5T32GM7288 from the NIH. C.S.R. is",

year = "1983",

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AU - Rubin, Charles S.

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