High-performance gel-permeation chromatography of polypeptides in a volatile solvent

Rapid resolution and molecular weight estimations of proteins and peptides on a column of TSK-G3000-PW

Gary D. Swergold, Charles S. Rubin

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

A gel-permeation column of TSK-G3000-PW that was equilibrated and developed with 36 or 45% acetonitrile in 0.1% trifluoroacetic acid fractionated mixtures of peptides with high resolving power. In addition, the elution volumes of 11 standard peptides and proteins were linearly related to the logarithms of their molecular weights in the acetonitrile-trifluoroacetic acid solvent at both low and high flow rates. Since the solvent is volatile and relatively transparent to short-wavelength ultraviolet light this high-performance gel-permeation system offers a rapid and highly sensitive method for the analysis, characterization, and purification of peptides and proteins from complex mixtures.

Original languageEnglish (US)
Pages (from-to)295-300
Number of pages6
JournalAnalytical Biochemistry
Volume131
Issue number2
DOIs
StatePublished - 1983

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Gel permeation chromatography
Gel Chromatography
Trifluoroacetic Acid
Molecular Weight
Molecular weight
Permeation
Peptides
Gels
Proteins
Optical resolving power
Ultraviolet Rays
Complex Mixtures
Purification
Flow rate
Wavelength
acetonitrile

Keywords

  • gel-permeation chromatography
  • peptide molecular weights

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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abstract = "A gel-permeation column of TSK-G3000-PW that was equilibrated and developed with 36 or 45{\%} acetonitrile in 0.1{\%} trifluoroacetic acid fractionated mixtures of peptides with high resolving power. In addition, the elution volumes of 11 standard peptides and proteins were linearly related to the logarithms of their molecular weights in the acetonitrile-trifluoroacetic acid solvent at both low and high flow rates. Since the solvent is volatile and relatively transparent to short-wavelength ultraviolet light this high-performance gel-permeation system offers a rapid and highly sensitive method for the analysis, characterization, and purification of peptides and proteins from complex mixtures.",
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T2 - Rapid resolution and molecular weight estimations of proteins and peptides on a column of TSK-G3000-PW

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AU - Rubin, Charles S.

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AB - A gel-permeation column of TSK-G3000-PW that was equilibrated and developed with 36 or 45% acetonitrile in 0.1% trifluoroacetic acid fractionated mixtures of peptides with high resolving power. In addition, the elution volumes of 11 standard peptides and proteins were linearly related to the logarithms of their molecular weights in the acetonitrile-trifluoroacetic acid solvent at both low and high flow rates. Since the solvent is volatile and relatively transparent to short-wavelength ultraviolet light this high-performance gel-permeation system offers a rapid and highly sensitive method for the analysis, characterization, and purification of peptides and proteins from complex mixtures.

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