High P-glycoprotein-mediated export observed in patients with a history of idiopathic thrombocytopenic purpura

Adam S. Levy, Susanna Cunningham-Rundles, BethAnne Mazza, Maciej Simm, Richard Gorlick, James Bussel

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Studies have suggested that high P-glycoprotein expression in lymphocytes from patients with autoimmune disorders may affect disease outcome. Idiopathic thrombocytopenic purpura (ITP) and Evans' syndrome are widely thought to be autoimmune processes, however, the precise mechanisms remain unknown. Peripheral blood mononuclear cells from patients with refractory or recurrent ITP or Evans' syndrome were studied using the rhodamine 123 flow cytometric assay to investigate functional export levels. Lymphocytes from ITP and Evans' syndrome patients showed a significantly decreased ability to retain rhodamine, suggesting increased export protein function. Reverse transcription polymerase chain reaction distinguished P-glycoprotein as the likely export protein.

Original languageEnglish (US)
Pages (from-to)836-838
Number of pages3
JournalBritish Journal of Haematology
Volume118
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Idiopathic Thrombocytopenic Purpura
P-Glycoprotein
Lymphocytes
Rhodamine 123
Rhodamines
Reverse Transcription
Blood Cells
Proteins
Polymerase Chain Reaction
Evans Syndrome

Keywords

  • Evans' syndrome
  • Idiopathic thrombocytopenic purpura
  • Multi-drug resistance
  • P-glycoprotein
  • Rhodamine 123

ASJC Scopus subject areas

  • Hematology

Cite this

High P-glycoprotein-mediated export observed in patients with a history of idiopathic thrombocytopenic purpura. / Levy, Adam S.; Cunningham-Rundles, Susanna; Mazza, BethAnne; Simm, Maciej; Gorlick, Richard; Bussel, James.

In: British Journal of Haematology, Vol. 118, No. 3, 2002, p. 836-838.

Research output: Contribution to journalArticle

Levy, Adam S. ; Cunningham-Rundles, Susanna ; Mazza, BethAnne ; Simm, Maciej ; Gorlick, Richard ; Bussel, James. / High P-glycoprotein-mediated export observed in patients with a history of idiopathic thrombocytopenic purpura. In: British Journal of Haematology. 2002 ; Vol. 118, No. 3. pp. 836-838.
@article{497af1259cd64518ae69b8315e4ad873,
title = "High P-glycoprotein-mediated export observed in patients with a history of idiopathic thrombocytopenic purpura",
abstract = "Studies have suggested that high P-glycoprotein expression in lymphocytes from patients with autoimmune disorders may affect disease outcome. Idiopathic thrombocytopenic purpura (ITP) and Evans' syndrome are widely thought to be autoimmune processes, however, the precise mechanisms remain unknown. Peripheral blood mononuclear cells from patients with refractory or recurrent ITP or Evans' syndrome were studied using the rhodamine 123 flow cytometric assay to investigate functional export levels. Lymphocytes from ITP and Evans' syndrome patients showed a significantly decreased ability to retain rhodamine, suggesting increased export protein function. Reverse transcription polymerase chain reaction distinguished P-glycoprotein as the likely export protein.",
keywords = "Evans' syndrome, Idiopathic thrombocytopenic purpura, Multi-drug resistance, P-glycoprotein, Rhodamine 123",
author = "Levy, {Adam S.} and Susanna Cunningham-Rundles and BethAnne Mazza and Maciej Simm and Richard Gorlick and James Bussel",
year = "2002",
doi = "10.1046/j.1365-2141.2002.03709.x",
language = "English (US)",
volume = "118",
pages = "836--838",
journal = "British Journal of Haematology",
issn = "0007-1048",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - High P-glycoprotein-mediated export observed in patients with a history of idiopathic thrombocytopenic purpura

AU - Levy, Adam S.

AU - Cunningham-Rundles, Susanna

AU - Mazza, BethAnne

AU - Simm, Maciej

AU - Gorlick, Richard

AU - Bussel, James

PY - 2002

Y1 - 2002

N2 - Studies have suggested that high P-glycoprotein expression in lymphocytes from patients with autoimmune disorders may affect disease outcome. Idiopathic thrombocytopenic purpura (ITP) and Evans' syndrome are widely thought to be autoimmune processes, however, the precise mechanisms remain unknown. Peripheral blood mononuclear cells from patients with refractory or recurrent ITP or Evans' syndrome were studied using the rhodamine 123 flow cytometric assay to investigate functional export levels. Lymphocytes from ITP and Evans' syndrome patients showed a significantly decreased ability to retain rhodamine, suggesting increased export protein function. Reverse transcription polymerase chain reaction distinguished P-glycoprotein as the likely export protein.

AB - Studies have suggested that high P-glycoprotein expression in lymphocytes from patients with autoimmune disorders may affect disease outcome. Idiopathic thrombocytopenic purpura (ITP) and Evans' syndrome are widely thought to be autoimmune processes, however, the precise mechanisms remain unknown. Peripheral blood mononuclear cells from patients with refractory or recurrent ITP or Evans' syndrome were studied using the rhodamine 123 flow cytometric assay to investigate functional export levels. Lymphocytes from ITP and Evans' syndrome patients showed a significantly decreased ability to retain rhodamine, suggesting increased export protein function. Reverse transcription polymerase chain reaction distinguished P-glycoprotein as the likely export protein.

KW - Evans' syndrome

KW - Idiopathic thrombocytopenic purpura

KW - Multi-drug resistance

KW - P-glycoprotein

KW - Rhodamine 123

UR - http://www.scopus.com/inward/record.url?scp=0036040063&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036040063&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2141.2002.03709.x

DO - 10.1046/j.1365-2141.2002.03709.x

M3 - Article

C2 - 12181055

AN - SCOPUS:0036040063

VL - 118

SP - 836

EP - 838

JO - British Journal of Haematology

JF - British Journal of Haematology

SN - 0007-1048

IS - 3

ER -