High P-glycoprotein-mediated export observed in patients with a history of idiopathic thrombocytopenic purpura

Adam S. Levy, Susanna Cunningham-Rundles, Beth Anne Mazza, Maciej Simm, Richard Gorlick, James Bussel

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Studies have suggested that high P-glycoprotein expression in lymphocytes from patients with autoimmune disorders may affect disease outcome. Idiopathic thrombocytopenic purpura (ITP) and Evans' syndrome are widely thought to be autoimmune processes, however, the precise mechanisms remain unknown. Peripheral blood mononuclear cells from patients with refractory or recurrent ITP or Evans' syndrome were studied using the rhodamine 123 flow cytometric assay to investigate functional export levels. Lymphocytes from ITP and Evans' syndrome patients showed a significantly decreased ability to retain rhodamine, suggesting increased export protein function. Reverse transcription polymerase chain reaction distinguished P-glycoprotein as the likely export protein.

Original languageEnglish (US)
Pages (from-to)836-838
Number of pages3
JournalBritish Journal of Haematology
Volume118
Issue number3
DOIs
StatePublished - Sep 23 2002

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Keywords

  • Evans' syndrome
  • Idiopathic thrombocytopenic purpura
  • Multi-drug resistance
  • P-glycoprotein
  • Rhodamine 123

ASJC Scopus subject areas

  • Hematology

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