High-Mobility group box 1 is dispensable for autophagy, mitochondrial quality control, and organ function in vivo

Peter Huebener, Geum Youn Gwak, Jean Philippe Pradere, Catarina M. Quinzii, Richard Friedman, Chyuan Sheng Lin, Chad M. Trent, Ingmar Mederacke, Enpeng Zhao, Dianne H. Dapito, Yuxi Lin, Ira J. Goldberg, Mark J. Czaja, Robert F. Schwabe

Research output: Contribution to journalArticle

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Abstract

In vitro studies have demonstrated a critical role for high-mobility group box 1 (HMGB1) in autophagy and the autophagic clearance of dysfunctional mitochondria, resulting in severe mitochondrial fragmentation and profound disturbances of mitochondrial respiration in HMGB1-deficient cells. Here, we investigated the effects of HMGB1 deficiency on autophagy and mitochondrial function in vivo, using conditional Hmgb1 ablation in the liver and heart. Unexpectedly, deletion of Hmgb1 in hepatocytes or cardiomyocytes, two cell types with abundant mitochondria, did not alter mitochondrial structure or function, organ function, or long-term survival. Moreover, hepatic autophagy and mitophagy occurred normally in the absence of Hmgb1, and absence of Hmgb1 did not significantly affect baseline and glucocorticoid-induced hepatic gene expression. Collectively, our findings suggest that HMGB1 is dispensable for autophagy, mitochondrial quality control, the regulation of gene expression, and organ function in the adult organism.

Original languageEnglish (US)
Pages (from-to)539-547
Number of pages9
JournalCell Metabolism
Volume19
Issue number3
DOIs
StatePublished - Mar 4 2014

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Autophagy
Quality Control
Liver
Mitochondria
Mitochondrial Degradation
Gene Expression Regulation
Cardiac Myocytes
Glucocorticoids
Hepatocytes
Respiration
Gene Expression

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Physiology

Cite this

Huebener, P., Gwak, G. Y., Pradere, J. P., Quinzii, C. M., Friedman, R., Lin, C. S., ... Schwabe, R. F. (2014). High-Mobility group box 1 is dispensable for autophagy, mitochondrial quality control, and organ function in vivo. Cell Metabolism, 19(3), 539-547. https://doi.org/10.1016/j.cmet.2014.01.014

High-Mobility group box 1 is dispensable for autophagy, mitochondrial quality control, and organ function in vivo. / Huebener, Peter; Gwak, Geum Youn; Pradere, Jean Philippe; Quinzii, Catarina M.; Friedman, Richard; Lin, Chyuan Sheng; Trent, Chad M.; Mederacke, Ingmar; Zhao, Enpeng; Dapito, Dianne H.; Lin, Yuxi; Goldberg, Ira J.; Czaja, Mark J.; Schwabe, Robert F.

In: Cell Metabolism, Vol. 19, No. 3, 04.03.2014, p. 539-547.

Research output: Contribution to journalArticle

Huebener, P, Gwak, GY, Pradere, JP, Quinzii, CM, Friedman, R, Lin, CS, Trent, CM, Mederacke, I, Zhao, E, Dapito, DH, Lin, Y, Goldberg, IJ, Czaja, MJ & Schwabe, RF 2014, 'High-Mobility group box 1 is dispensable for autophagy, mitochondrial quality control, and organ function in vivo', Cell Metabolism, vol. 19, no. 3, pp. 539-547. https://doi.org/10.1016/j.cmet.2014.01.014
Huebener, Peter ; Gwak, Geum Youn ; Pradere, Jean Philippe ; Quinzii, Catarina M. ; Friedman, Richard ; Lin, Chyuan Sheng ; Trent, Chad M. ; Mederacke, Ingmar ; Zhao, Enpeng ; Dapito, Dianne H. ; Lin, Yuxi ; Goldberg, Ira J. ; Czaja, Mark J. ; Schwabe, Robert F. / High-Mobility group box 1 is dispensable for autophagy, mitochondrial quality control, and organ function in vivo. In: Cell Metabolism. 2014 ; Vol. 19, No. 3. pp. 539-547.
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