TY - JOUR
T1 - High levels of IL-4 and IL-10 mRNA and low levels of IL-2, IL-9 and IFN- γ mRNA in MuLV-induced lymphomas
AU - Beaty, R. M.
AU - Rulli, K.
AU - Bost, K. L.
AU - Pantginis, J.
AU - Lenz, J.
AU - Levy, L. S.
N1 - Funding Information:
The authors gratefully acknowledge the assistance of Christal Bowman with studies of the IL-9R locus in tumor DNA. This work was supported by grants from the American Cancer Society (RPG-94–012-04-VM) and the Ladies Leukemia League to L.S.L. and by Development Funds of the Tulane Cancer Center. J.L. was supported by National Institutes of Health Grants CA-44822 and CA-57337. J.P. was supported by NIH Training Grant CA-09060.
PY - 1999/9/1
Y1 - 1999/9/1
N2 - The expression of cytokines may influence the development of lymphoma in retrovirally infected animals in at least two ways: (1) cytokines in the tumor environment may stimulate the proliferation of tumor cells and/or (2)cytokines in the tumor environment may diminish the cell-mediated antitumor immune response. To evaluate these possibilities, a semiquantitative RT-POR approach was utilized to permit a broad screening of cytokine mRNAs in a large number of tissue samples. Examination of MuLV- induced end-stage lymphomas revealed the absence of mRNA for cytokines known to stimulate the proliferation of T cells (i.e., IL-2, IL-9), the absence of mRNA for cytokines known to enhance cell-mediated antitumor immune responses (i.e., IL-2, IFNγ,), and the presence of mRNA for cytokines known to diminish such responses (i.e., IL-4, IL-10). Similar patterns of cytokine mRNA expression were detected in tumor-derived cell lines. Spleen and thymus from animals collected longitudinally during infection and from age-matched uninfected mice also demonstrated a similar pattern, except that IFNγ, mRNA was readily detectable. These findings do not support the hypothesis that the developing tumor depends on cytokines to provide proliferative signals. The findings suggest that cytokines in the immediate environment of the lymphoma support tumor development by acting to diminish an effective antitumor immune response.
AB - The expression of cytokines may influence the development of lymphoma in retrovirally infected animals in at least two ways: (1) cytokines in the tumor environment may stimulate the proliferation of tumor cells and/or (2)cytokines in the tumor environment may diminish the cell-mediated antitumor immune response. To evaluate these possibilities, a semiquantitative RT-POR approach was utilized to permit a broad screening of cytokine mRNAs in a large number of tissue samples. Examination of MuLV- induced end-stage lymphomas revealed the absence of mRNA for cytokines known to stimulate the proliferation of T cells (i.e., IL-2, IL-9), the absence of mRNA for cytokines known to enhance cell-mediated antitumor immune responses (i.e., IL-2, IFNγ,), and the presence of mRNA for cytokines known to diminish such responses (i.e., IL-4, IL-10). Similar patterns of cytokine mRNA expression were detected in tumor-derived cell lines. Spleen and thymus from animals collected longitudinally during infection and from age-matched uninfected mice also demonstrated a similar pattern, except that IFNγ, mRNA was readily detectable. These findings do not support the hypothesis that the developing tumor depends on cytokines to provide proliferative signals. The findings suggest that cytokines in the immediate environment of the lymphoma support tumor development by acting to diminish an effective antitumor immune response.
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U2 - 10.1006/viro.1999.9846
DO - 10.1006/viro.1999.9846
M3 - Article
C2 - 10497110
AN - SCOPUS:0033199297
SN - 0042-6822
VL - 261
SP - 253
EP - 262
JO - Virology
JF - Virology
IS - 2
ER -