High-frequency transfection of CHO cells using polybrene

William G. Chaney, Daniel R. Howard, Jeffrey W. Pollard, Sandra Sallustio, Pamela Stanley

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

High-frequency transfection of CHO cells has been achieved for several plasmids, a cosmid library, and genomic DNA using Polybrene and dimethyl sulfoxide. All plasmid transfectants examined were stable and exhibited plasmid sequences in genomic DNA. The method is simple, reproducible, and succeeded with several independent CHO clones in the presence or the absence of carrier DNA, even at very low concentrations of plasmid DNA.

Original languageEnglish (US)
Pages (from-to)237-244
Number of pages8
JournalSomatic Cell and Molecular Genetics
Volume12
Issue number3
DOIs
StatePublished - May 1986

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Hexadimethrine Bromide
CHO Cells
Transfection
Plasmids
DNA
Cosmids
Genomic Library
Dimethyl Sulfoxide
Gene Library
Clone Cells

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

High-frequency transfection of CHO cells using polybrene. / Chaney, William G.; Howard, Daniel R.; Pollard, Jeffrey W.; Sallustio, Sandra; Stanley, Pamela.

In: Somatic Cell and Molecular Genetics, Vol. 12, No. 3, 05.1986, p. 237-244.

Research output: Contribution to journalArticle

Chaney, William G. ; Howard, Daniel R. ; Pollard, Jeffrey W. ; Sallustio, Sandra ; Stanley, Pamela. / High-frequency transfection of CHO cells using polybrene. In: Somatic Cell and Molecular Genetics. 1986 ; Vol. 12, No. 3. pp. 237-244.
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