High expression of macrophage migration inhibitory factor in the murine lacrimal gland

Choul Yong Park, Young Joo Shin, Kaevalin Lekhanont, Cheng Zhang, Woo Seok Lee, Marisol Cano, Richard Bucala, Roy S. Chuck

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: To report the expression of macrophage migration inhibitory factor (MIF) in murine lacrimal glands. Methods: Six lacrimal glands from 3 female 8-week-old CBA/J mice were analyzed using a gene microarray method (Oligo GEArray mouse inflammatory cytokines and receptors, Catalog No. OMM-011, SuperArray Bioscience) and immunofluorescent staining for MIF. Nine lacrimal glands from 8-week-old CBA/J mice, 9 lacrimal glands from 8-week-old C57BL/6 mice, and 7 lacrimal glands from 8-week-old BALB/c mice were analyzed using real-time reverse transcription-polymerase chain reaction. Five spleens, 5 livers, and 5 serum samples from 5 female 8-week-old CBA/J mice were analyzed using enzyme-linked immunosorbent assay. Results: Microarray analysis revealed that MIF is highly expressed in the murine lacrimal gland. Lacrimal acinar cells stain strongly with anti-MIF antibodies. Real-time reverse transcription- polymerase chain reaction revealed that the MIF mRNA expression level is lower in lacrimal glands of CBA/J mice compared with C57BL/6 and BALB/c mice when normalized against the expression of β-actin mRNA. Enzyme-linked immunosorbent assay revealed that MIF level was higher in lacrimal glands and spleens compared with livers and sera. Conclusions: The murine lacrimal gland expresses high levels of macrophage MIF without any evidence of lacrimal gland inflammation.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalCornea
Volume29
Issue number2
DOIs
StatePublished - Feb 2010
Externally publishedYes

Fingerprint

Macrophage Migration-Inhibitory Factors
Lacrimal Apparatus
Inbred CBA Mouse
Reverse Transcription
Spleen
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction
Messenger RNA
Cytokine Receptors
Acinar Cells
Liver
Microarray Analysis
Serum
Tears
Inbred C57BL Mouse
Actins
Coloring Agents
Staining and Labeling

Keywords

  • Dry eye
  • Dysfunctional tear
  • Inflammation
  • Inhibition
  • Lacrimal gland
  • Macrophage
  • MIF
  • Mouse
  • Ocular surface

ASJC Scopus subject areas

  • Ophthalmology

Cite this

High expression of macrophage migration inhibitory factor in the murine lacrimal gland. / Yong Park, Choul; Shin, Young Joo; Lekhanont, Kaevalin; Zhang, Cheng; Lee, Woo Seok; Cano, Marisol; Bucala, Richard; Chuck, Roy S.

In: Cornea, Vol. 29, No. 2, 02.2010, p. 187-191.

Research output: Contribution to journalArticle

Yong Park, C, Shin, YJ, Lekhanont, K, Zhang, C, Lee, WS, Cano, M, Bucala, R & Chuck, RS 2010, 'High expression of macrophage migration inhibitory factor in the murine lacrimal gland', Cornea, vol. 29, no. 2, pp. 187-191. https://doi.org/10.1097/ICO.0b013e3181c06466
Yong Park, Choul ; Shin, Young Joo ; Lekhanont, Kaevalin ; Zhang, Cheng ; Lee, Woo Seok ; Cano, Marisol ; Bucala, Richard ; Chuck, Roy S. / High expression of macrophage migration inhibitory factor in the murine lacrimal gland. In: Cornea. 2010 ; Vol. 29, No. 2. pp. 187-191.
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abstract = "Purpose: To report the expression of macrophage migration inhibitory factor (MIF) in murine lacrimal glands. Methods: Six lacrimal glands from 3 female 8-week-old CBA/J mice were analyzed using a gene microarray method (Oligo GEArray mouse inflammatory cytokines and receptors, Catalog No. OMM-011, SuperArray Bioscience) and immunofluorescent staining for MIF. Nine lacrimal glands from 8-week-old CBA/J mice, 9 lacrimal glands from 8-week-old C57BL/6 mice, and 7 lacrimal glands from 8-week-old BALB/c mice were analyzed using real-time reverse transcription-polymerase chain reaction. Five spleens, 5 livers, and 5 serum samples from 5 female 8-week-old CBA/J mice were analyzed using enzyme-linked immunosorbent assay. Results: Microarray analysis revealed that MIF is highly expressed in the murine lacrimal gland. Lacrimal acinar cells stain strongly with anti-MIF antibodies. Real-time reverse transcription- polymerase chain reaction revealed that the MIF mRNA expression level is lower in lacrimal glands of CBA/J mice compared with C57BL/6 and BALB/c mice when normalized against the expression of β-actin mRNA. Enzyme-linked immunosorbent assay revealed that MIF level was higher in lacrimal glands and spleens compared with livers and sera. Conclusions: The murine lacrimal gland expresses high levels of macrophage MIF without any evidence of lacrimal gland inflammation.",
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AU - Shin, Young Joo

AU - Lekhanont, Kaevalin

AU - Zhang, Cheng

AU - Lee, Woo Seok

AU - Cano, Marisol

AU - Bucala, Richard

AU - Chuck, Roy S.

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N2 - Purpose: To report the expression of macrophage migration inhibitory factor (MIF) in murine lacrimal glands. Methods: Six lacrimal glands from 3 female 8-week-old CBA/J mice were analyzed using a gene microarray method (Oligo GEArray mouse inflammatory cytokines and receptors, Catalog No. OMM-011, SuperArray Bioscience) and immunofluorescent staining for MIF. Nine lacrimal glands from 8-week-old CBA/J mice, 9 lacrimal glands from 8-week-old C57BL/6 mice, and 7 lacrimal glands from 8-week-old BALB/c mice were analyzed using real-time reverse transcription-polymerase chain reaction. Five spleens, 5 livers, and 5 serum samples from 5 female 8-week-old CBA/J mice were analyzed using enzyme-linked immunosorbent assay. Results: Microarray analysis revealed that MIF is highly expressed in the murine lacrimal gland. Lacrimal acinar cells stain strongly with anti-MIF antibodies. Real-time reverse transcription- polymerase chain reaction revealed that the MIF mRNA expression level is lower in lacrimal glands of CBA/J mice compared with C57BL/6 and BALB/c mice when normalized against the expression of β-actin mRNA. Enzyme-linked immunosorbent assay revealed that MIF level was higher in lacrimal glands and spleens compared with livers and sera. Conclusions: The murine lacrimal gland expresses high levels of macrophage MIF without any evidence of lacrimal gland inflammation.

AB - Purpose: To report the expression of macrophage migration inhibitory factor (MIF) in murine lacrimal glands. Methods: Six lacrimal glands from 3 female 8-week-old CBA/J mice were analyzed using a gene microarray method (Oligo GEArray mouse inflammatory cytokines and receptors, Catalog No. OMM-011, SuperArray Bioscience) and immunofluorescent staining for MIF. Nine lacrimal glands from 8-week-old CBA/J mice, 9 lacrimal glands from 8-week-old C57BL/6 mice, and 7 lacrimal glands from 8-week-old BALB/c mice were analyzed using real-time reverse transcription-polymerase chain reaction. Five spleens, 5 livers, and 5 serum samples from 5 female 8-week-old CBA/J mice were analyzed using enzyme-linked immunosorbent assay. Results: Microarray analysis revealed that MIF is highly expressed in the murine lacrimal gland. Lacrimal acinar cells stain strongly with anti-MIF antibodies. Real-time reverse transcription- polymerase chain reaction revealed that the MIF mRNA expression level is lower in lacrimal glands of CBA/J mice compared with C57BL/6 and BALB/c mice when normalized against the expression of β-actin mRNA. Enzyme-linked immunosorbent assay revealed that MIF level was higher in lacrimal glands and spleens compared with livers and sera. Conclusions: The murine lacrimal gland expresses high levels of macrophage MIF without any evidence of lacrimal gland inflammation.

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KW - Ocular surface

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