High Dead Volume Pumps: Delay Time Reduction by Short-Term Low-Ratio Split Flow in LC/MS Applications

Eduard Rogatsky, Daniel T. Stein

Research output: Contribution to journalArticle

1 Scopus citations


The aim of our work was to develop a low-cost, simple and reliable solution to reduce LC/MS analysis time by compensating for limitations inherent to high dead volume standard HPLC pumps. In our approach, we utilized a temporary (sub-1 min) low ratio flow split (∼1:10) at 5 mL/min pump flow before the column. During this short period, 90% of the entire pump flow is delivered to waste and used for fast pump/system equilibration. Although full-time flow splitting is widely used in capillary/nano applications (usually with high split ratios in the hundreds or thousands), to our knowledge, this is the first time that short-term low-ratio flow splitting has been used in conventional LC/MS applications.

Original languageEnglish (US)
Pages (from-to)245-247
Number of pages3
JournalJournal of the American Society for Mass Spectrometry
Issue number2
Publication statusPublished - Feb 1 2007


ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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