TY - JOUR
T1 - High Dead Volume Pumps
T2 - Delay Time Reduction by Short-Term Low-Ratio Split Flow in LC/MS Applications
AU - Rogatsky, Eduard
AU - Stein, Daniel T.
N1 - Funding Information:
This research was supported by grants from the American Diabetes Association, NIH/NCRR and NIDDK: MO1-RR12248 (GCRC) and R01 DK61644-01.
PY - 2007/2
Y1 - 2007/2
N2 - The aim of our work was to develop a low-cost, simple and reliable solution to reduce LC/MS analysis time by compensating for limitations inherent to high dead volume standard HPLC pumps. In our approach, we utilized a temporary (sub-1 min) low ratio flow split (∼1:10) at 5 mL/min pump flow before the column. During this short period, 90% of the entire pump flow is delivered to waste and used for fast pump/system equilibration. Although full-time flow splitting is widely used in capillary/nano applications (usually with high split ratios in the hundreds or thousands), to our knowledge, this is the first time that short-term low-ratio flow splitting has been used in conventional LC/MS applications.
AB - The aim of our work was to develop a low-cost, simple and reliable solution to reduce LC/MS analysis time by compensating for limitations inherent to high dead volume standard HPLC pumps. In our approach, we utilized a temporary (sub-1 min) low ratio flow split (∼1:10) at 5 mL/min pump flow before the column. During this short period, 90% of the entire pump flow is delivered to waste and used for fast pump/system equilibration. Although full-time flow splitting is widely used in capillary/nano applications (usually with high split ratios in the hundreds or thousands), to our knowledge, this is the first time that short-term low-ratio flow splitting has been used in conventional LC/MS applications.
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U2 - 10.1016/j.jasms.2006.09.019
DO - 10.1016/j.jasms.2006.09.019
M3 - Article
C2 - 17095244
AN - SCOPUS:33846439741
SN - 1044-0305
VL - 18
SP - 245
EP - 247
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
IS - 2
ER -