Hexameric assembly of the proteasomal ATPases is templated through their C termini

Soyeon Park, Jeroen Roelofs, Woong Kim, Jessica Robert, Marion Schmidt, Steven P. Gygi, Daniel Finley

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Substrates of the proteasome are recognized and unfolded by the regulatory particle, and then translocated into the core particle (CP) to be degraded. A hetero-hexameric ATPase ring, containing subunits Rpt1-6, is situated within the base subassembly of the regulatory particle. The ATPase ring sits atop the CP, with the Rpt carboxy termini inserted into pockets in the CP. Here we identify a previously unknown function of the Rpt proteins in proteasome biogenesis through deleting the C-terminal residue from each Rpt in the yeast Saccharomyces cerevisiae. Our results indicate that assembly of the hexameric ATPase ring is templated on the CP. We have also identified an apparent intermediate in base assembly, BP1, which contains Rpn1, three Rpts and Hsm3, a chaperone for base assembly. The Rpt proteins with the strongest assembly phenotypes, Rpt4 and Rpt6, were absent from BP1. We propose that Rpt4 and Rpt6 form a nucleating complex to initiate base assembly, and that this complex is subsequently joined by BP1 to complete the Rpt ring. Our studies show that assembly of the proteasome base is a rapid yet highly orchestrated process.

Original languageEnglish (US)
Pages (from-to)866-870
Number of pages5
JournalNature
Volume459
Issue number7248
DOIs
StatePublished - Jun 11 2009

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Proteasome Endopeptidase Complex
Adenosine Triphosphatases
Saccharomyces cerevisiae
Proteins
Yeasts
Phenotype

ASJC Scopus subject areas

  • General

Cite this

Park, S., Roelofs, J., Kim, W., Robert, J., Schmidt, M., Gygi, S. P., & Finley, D. (2009). Hexameric assembly of the proteasomal ATPases is templated through their C termini. Nature, 459(7248), 866-870. https://doi.org/10.1038/nature08065

Hexameric assembly of the proteasomal ATPases is templated through their C termini. / Park, Soyeon; Roelofs, Jeroen; Kim, Woong; Robert, Jessica; Schmidt, Marion; Gygi, Steven P.; Finley, Daniel.

In: Nature, Vol. 459, No. 7248, 11.06.2009, p. 866-870.

Research output: Contribution to journalArticle

Park, S, Roelofs, J, Kim, W, Robert, J, Schmidt, M, Gygi, SP & Finley, D 2009, 'Hexameric assembly of the proteasomal ATPases is templated through their C termini', Nature, vol. 459, no. 7248, pp. 866-870. https://doi.org/10.1038/nature08065
Park S, Roelofs J, Kim W, Robert J, Schmidt M, Gygi SP et al. Hexameric assembly of the proteasomal ATPases is templated through their C termini. Nature. 2009 Jun 11;459(7248):866-870. https://doi.org/10.1038/nature08065
Park, Soyeon ; Roelofs, Jeroen ; Kim, Woong ; Robert, Jessica ; Schmidt, Marion ; Gygi, Steven P. ; Finley, Daniel. / Hexameric assembly of the proteasomal ATPases is templated through their C termini. In: Nature. 2009 ; Vol. 459, No. 7248. pp. 866-870.
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