Heterotypic gap junction channels (connexin26 - connexin32) violate the paradigm of unitary conductance

Feliksas F. Bukauskas, Claudia Elfgang, Klaus Willecke, Robert Weingart

Research output: Contribution to journalArticle

94 Scopus citations

Abstract

Human HeLa cells transfected with mouse DNA coding for connexin26 (Cx26) or connexin32 (Cx32) were used to examine the properties of heterotypic Cx26 - Cx32 gap junction channels. Intercellular current flow was examined in induced cell pairs by means of the dual voltage-clamp method. We found that Cx26 - Cx32 channels exhibit voltage-dependent conductances, γj: γj(main state) increases with increasing positivity at the cytoplasmic aspect of the Cx26 connexon and decreases with increasing negativity (slope: 32 pS/100 mV; γj γj(main state) reaches 48 pS as V j approaches 0 mV); γj(residual state) with a similar Vj-dependence is present when the cytoplasmic end of Cx26 connexon is positive, but absent when it is negative. The single channel data provide an explanation for the asymmetric relationships between the gap junction conductance, gj, and Vj. The results are consistent with the notion that docking of two connexons co-determines the biophysical properties of a gap junction channel.

Original languageEnglish (US)
Pages (from-to)870-872
Number of pages3
JournalPflügers Archiv European Journal of Physiology
Volume429
Issue number6
DOIs
StatePublished - Apr 1 1995

Keywords

  • HeLa cells
  • connexin26
  • connexin32
  • gap junction channels
  • heterotypic channels
  • single channel conductance

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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