Heteronuclear two‐dimensional 15N‐ and 13C‐nmr studies of DNA oligomers and their netropsin complexes using indirect proton detection

Joseph Ashcroft, David H. Live, Dinshaw J. Patel, David Cowburn

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Heteronuclear multispin coherence proton‐detected two‐dimensional nmr spectroscopic experiments were used to obtain information on protonated carbons and nitrogens of the self‐complementary d (G‐G‐T‐A‐T‐A‐C‐C) and d (G‐G‐A‐A‐T‐T‐C‐C) duplexes, with and without the drug netropsin dissolved in aqueous solution. Many correlations of protons coupled to 13C nuclei on the base and sugar rings of the octanucleotides were detected, allowing the carbon resonances to be assigned based on previous homonuclear proton two‐dimensional nmr studies. Imino nitrogen assignments can also be made using the proton assignments from previous one‐dimensional nuclear overhauser effect experiments. Imino nitrogen shifts may be useful indicators of changes in local hydrogen‐bonding interactions to base‐pair edges.

Original languageEnglish (US)
Pages (from-to)45-55
Number of pages11
JournalBiopolymers
Volume31
Issue number1
DOIs
StatePublished - Jan 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

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