Herpes simplex virus capsid-organelle association in the absence of the large tegument protein UL36p

Himanshu Kharkwal, Sara Shanda Furgiuele, Caitlin G. Smith, Duncan W. Wilson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

UL36p (VP1/2) is the largest protein encoded by herpes simplex virus 1 (HSV-1) and resides in the innermost layer of the viral tegument, lying between the capsid and the envelope. UL36p performs multiple functions in the HSV life cycle, including an essential role in cytoplasmic envelopment. We earlier described the isolation of a virion-associated cytoplasmic membrane fraction from HSV-infected cells. Biochemical and ultrastructural analyses showed that the organelles in this buoyant fraction contain enveloped infectious HSV particles in their lumens and naked capsids docked to their cytoplasmic surfaces. These organelles can also recruit molecular motors and transport their cargo virions along microtubules in vitro. Here we examine the properties of these HSV-associated organelles in the absence of UL36p. We find that while capsid envelopment is clearly defective, a subpopulation of capsids nevertheless still associate with the cytoplasmic faces of these organelles. The existence of these capsidmembrane structures was confirmed by subcellular fractionation, immunocytochemistry, lipophilic dye fluorescence microscopy, thin-section electron microscopy, and correlative light and electron microscopy. We conclude that capsid-membrane binding can occur in the absence of UL36p and propose that this association may precede the events of UL36p-driven envelopment.

Original languageEnglish (US)
Pages (from-to)11372-11382
Number of pages11
JournalJournal of Virology
Volume89
Issue number22
DOIs
StatePublished - 2015

Fingerprint

herpes simplex
capsid
Capsid
Simplexvirus
Organelles
organelles
viruses
Proteins
virion
proteins
Virion
electron microscopy
Electron Microscopy
Human herpesvirus 1
Human Herpesvirus 1
immunocytochemistry
Life Cycle Stages
fluorescence microscopy
Fluorescence Microscopy
Microtubules

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Herpes simplex virus capsid-organelle association in the absence of the large tegument protein UL36p. / Kharkwal, Himanshu; Furgiuele, Sara Shanda; Smith, Caitlin G.; Wilson, Duncan W.

In: Journal of Virology, Vol. 89, No. 22, 2015, p. 11372-11382.

Research output: Contribution to journalArticle

Kharkwal, Himanshu ; Furgiuele, Sara Shanda ; Smith, Caitlin G. ; Wilson, Duncan W. / Herpes simplex virus capsid-organelle association in the absence of the large tegument protein UL36p. In: Journal of Virology. 2015 ; Vol. 89, No. 22. pp. 11372-11382.
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