Hepatitis B virus DNA contains a glucocorticoid-responsive element

R. Tur-Kaspa, Robert D. Burk, Y. Shaul, D. A. Shafritz

Research output: Contribution to journalArticle

315 Citations (Scopus)

Abstract

It has recently been shown that hepatitis B virus (HBV) contains a transcriptional enhancer element. In order to determine whether this enhancer responds to glucocorticoids, a series of derivatives of plasmid pA10CAT2 was constructed containing the HBV enhancer and variable lengths of further upstream sequences. Transient expression of chloramphenicol acetyltransferase (CAT) was determined after introduction of these plasmids into PLC/PRF/5, Hep 3B, Hep G2, HeLa, and mouse L cells, Highest CAT activity was noted in the human hepatocellular carcinoma line PLC/PRF/5, which contains integrated HBV DNA sequences. Dexamethasone augmented CAT expression in all cell lines tested with 40% of maximal induction at 10 nM and maximum stimulation (3- to 8-fold) at 1 μM dexamethasone. Desamethasone augmentation of CAT expression was observed only when constructs contained HBV DNA sequences residing upstream to map position 735 from the EcoRI site. This indicates that the glucocorticoid-responsive region is distinct from the previously defined HBV enhancer sequence located at map position 1080-1234. These studies suggest that HBV DNA contains a glucocorticoid-responsive element, which may mediate expression of HBV genes in infected mammalian cells.

Original languageEnglish (US)
Pages (from-to)1627-1631
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number6
StatePublished - 1986

Fingerprint

Hepatitis B virus
Glucocorticoids
Chloramphenicol O-Acetyltransferase
DNA
Dexamethasone
Plasmids
Hepatocellular Carcinoma
Cell Line
Genes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Hepatitis B virus DNA contains a glucocorticoid-responsive element. / Tur-Kaspa, R.; Burk, Robert D.; Shaul, Y.; Shafritz, D. A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 6, 1986, p. 1627-1631.

Research output: Contribution to journalArticle

@article{81ad2c38e92445bbb4009ee02958a881,
title = "Hepatitis B virus DNA contains a glucocorticoid-responsive element",
abstract = "It has recently been shown that hepatitis B virus (HBV) contains a transcriptional enhancer element. In order to determine whether this enhancer responds to glucocorticoids, a series of derivatives of plasmid pA10CAT2 was constructed containing the HBV enhancer and variable lengths of further upstream sequences. Transient expression of chloramphenicol acetyltransferase (CAT) was determined after introduction of these plasmids into PLC/PRF/5, Hep 3B, Hep G2, HeLa, and mouse L cells, Highest CAT activity was noted in the human hepatocellular carcinoma line PLC/PRF/5, which contains integrated HBV DNA sequences. Dexamethasone augmented CAT expression in all cell lines tested with 40{\%} of maximal induction at 10 nM and maximum stimulation (3- to 8-fold) at 1 μM dexamethasone. Desamethasone augmentation of CAT expression was observed only when constructs contained HBV DNA sequences residing upstream to map position 735 from the EcoRI site. This indicates that the glucocorticoid-responsive region is distinct from the previously defined HBV enhancer sequence located at map position 1080-1234. These studies suggest that HBV DNA contains a glucocorticoid-responsive element, which may mediate expression of HBV genes in infected mammalian cells.",
author = "R. Tur-Kaspa and Burk, {Robert D.} and Y. Shaul and Shafritz, {D. A.}",
year = "1986",
language = "English (US)",
volume = "83",
pages = "1627--1631",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "6",

}

TY - JOUR

T1 - Hepatitis B virus DNA contains a glucocorticoid-responsive element

AU - Tur-Kaspa, R.

AU - Burk, Robert D.

AU - Shaul, Y.

AU - Shafritz, D. A.

PY - 1986

Y1 - 1986

N2 - It has recently been shown that hepatitis B virus (HBV) contains a transcriptional enhancer element. In order to determine whether this enhancer responds to glucocorticoids, a series of derivatives of plasmid pA10CAT2 was constructed containing the HBV enhancer and variable lengths of further upstream sequences. Transient expression of chloramphenicol acetyltransferase (CAT) was determined after introduction of these plasmids into PLC/PRF/5, Hep 3B, Hep G2, HeLa, and mouse L cells, Highest CAT activity was noted in the human hepatocellular carcinoma line PLC/PRF/5, which contains integrated HBV DNA sequences. Dexamethasone augmented CAT expression in all cell lines tested with 40% of maximal induction at 10 nM and maximum stimulation (3- to 8-fold) at 1 μM dexamethasone. Desamethasone augmentation of CAT expression was observed only when constructs contained HBV DNA sequences residing upstream to map position 735 from the EcoRI site. This indicates that the glucocorticoid-responsive region is distinct from the previously defined HBV enhancer sequence located at map position 1080-1234. These studies suggest that HBV DNA contains a glucocorticoid-responsive element, which may mediate expression of HBV genes in infected mammalian cells.

AB - It has recently been shown that hepatitis B virus (HBV) contains a transcriptional enhancer element. In order to determine whether this enhancer responds to glucocorticoids, a series of derivatives of plasmid pA10CAT2 was constructed containing the HBV enhancer and variable lengths of further upstream sequences. Transient expression of chloramphenicol acetyltransferase (CAT) was determined after introduction of these plasmids into PLC/PRF/5, Hep 3B, Hep G2, HeLa, and mouse L cells, Highest CAT activity was noted in the human hepatocellular carcinoma line PLC/PRF/5, which contains integrated HBV DNA sequences. Dexamethasone augmented CAT expression in all cell lines tested with 40% of maximal induction at 10 nM and maximum stimulation (3- to 8-fold) at 1 μM dexamethasone. Desamethasone augmentation of CAT expression was observed only when constructs contained HBV DNA sequences residing upstream to map position 735 from the EcoRI site. This indicates that the glucocorticoid-responsive region is distinct from the previously defined HBV enhancer sequence located at map position 1080-1234. These studies suggest that HBV DNA contains a glucocorticoid-responsive element, which may mediate expression of HBV genes in infected mammalian cells.

UR - http://www.scopus.com/inward/record.url?scp=2642589461&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2642589461&partnerID=8YFLogxK

M3 - Article

VL - 83

SP - 1627

EP - 1631

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 6

ER -