Hemopressins and other hemoglobin-derived peptides in mouse brain: Comparison between brain, blood, and heart peptidome and regulation in Cpe fat/fat mice

Julia S. Gelman, Juan Sironi, Leandro M. Castro, Emer S. Ferro, Lloyd D. Fricker

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Many hemoglobin-derived peptides are present in mouse brain, and several of these have bioactive properties including the hemopressins, a related series of peptides that bind to cannabinoid CB1 receptors. Although hemoglobin is a major component of red blood cells, it is also present in neurons and glia. To examine whether the hemoglobin-derived peptides in brain are similar to those present in blood and heart, we used a peptidomics approach involving mass spectrometry. Many hemoglobin-derived peptides are found only in brain and not in blood, whereas all hemoglobin-derived peptides found in heart were also seen in blood. Thus, it is likely that the majority of the hemoglobin-derived peptides detected in brain are produced from brain hemoglobin and not erythrocytes. We also examined if the hemopressins and other major hemoglobin-derived peptides were regulated in the Cpefat/fat mouse; previously these mice were reported to have elevated levels of several hemoglobin-derived peptides. Many, but not all of the hemoglobin-derived peptides were elevated in several brain regions of the Cpefat/fat mouse. Taken together, these findings suggest that the post-translational processing of alpha and beta hemoglobin into the hemopressins, as well as other peptides, is up-regulated in some but not all Cpefat/fat mouse brain regions.

Original languageEnglish (US)
Pages (from-to)871-880
Number of pages10
JournalJournal of Neurochemistry
Volume113
Issue number4
DOIs
StatePublished - May 1 2010

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Keywords

  • Cannabinoid
  • Carboxypeptidase E
  • Hemoglobin
  • Neuropeptide
  • Peptide processing

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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