Hemolytic and genotoxic evaluation of organochalcogens in human blood cells in vitro

D. B. Santos, V. P P Schiar, M. W. Paixão, D. F. Meinerz, C. W. Nogueira, Michael Aschner, J. B T Rocha, N. B V Barbosa

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

This study investigated the hemolytic and genotoxic effect of different organoselenium and organotellurium compounds in human blood cells, as simple tests for screening the toxicity of organochalcogenides. For osmotic fragility (OF) test, samples of total blood were incubated with the organochalcogens at 4, 8, 50, 75 and 100 μM or vehicle (DMSO) for 90 min at 37 °C. The EC50 values for hemolysis were significantly increased in erythrocytes exposed to diphenyl selenide (II), diphenyl diselenide (III), diphenyl telluride (IV), diphenyl ditelluride (V), (S)-2-amino-1-diselenide-3-methylpropanyl (IX), butyl(styryl)telluride (XIII) and 2-(butyltellurium)furan (XIV) at higher concentrations tested. The exposure of erythrocytes to organochalcogens diphenyl diselenide (II) and butyl(styryl)telluride (XIII), which had greater hemolytic effect, did not modify catalase activity, reactive oxygen species (ROS) production and -SH content. On the other hand, Na+/K+ ATPase activity of erythrocyte ghosts was significantly inhibited by the compounds diphenyl diselenide (II) and butyl(styryl)telluride (XIII) (P < 0.05) in a concentration-dependent manner. The inhibition of Na+/K+ ATPase activity was completely reversed by dithiothreitol (DTT); indicating reaction of these organochalcogens with thiol groups of the enzyme. The thiol oxidase activity of the compounds II and XIII was supported by the fact that the rate of DTT oxidation was increased significantly by both chalcogens. In the higher concentrations, the compounds (II) and (XIII) were strongly genotoxic and cytotoxic to human leukocytes cells, as verified by the DNA damage and cell viability evaluation. Our results suggest that at relatively high concentration organochalcogenides exhibit hemolytic and genotoxic action in human blood cells, which are probably linked to their thiol oxidase activity and preferential interaction with sulfhydryl groups critical to enzymes.

Original languageEnglish (US)
Pages (from-to)1195-1204
Number of pages10
JournalToxicology in Vitro
Volume23
Issue number6
DOIs
StatePublished - Sep 2009
Externally publishedYes

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Sulfhydryl Compounds
Blood Cells
Blood
Dithiothreitol
Cells
Adenosine Triphosphatases
Oxidoreductases
Organoselenium Compounds
Chalcogens
Erythrocytes
Osmotic Fragility
Toxicity Tests
Erythrocyte Membrane
Enzymes
Hemolysis
Dimethyl Sulfoxide
Catalase
DNA Damage
Toxicity
Reactive Oxygen Species

Keywords

  • DNA damage
  • Erythrocytes
  • Na/K ATPase
  • Osmotic fragility
  • Selenium
  • Tellurium

ASJC Scopus subject areas

  • Toxicology

Cite this

Santos, D. B., Schiar, V. P. P., Paixão, M. W., Meinerz, D. F., Nogueira, C. W., Aschner, M., ... Barbosa, N. B. V. (2009). Hemolytic and genotoxic evaluation of organochalcogens in human blood cells in vitro. Toxicology in Vitro, 23(6), 1195-1204. https://doi.org/10.1016/j.tiv.2009.05.010

Hemolytic and genotoxic evaluation of organochalcogens in human blood cells in vitro. / Santos, D. B.; Schiar, V. P P; Paixão, M. W.; Meinerz, D. F.; Nogueira, C. W.; Aschner, Michael; Rocha, J. B T; Barbosa, N. B V.

In: Toxicology in Vitro, Vol. 23, No. 6, 09.2009, p. 1195-1204.

Research output: Contribution to journalArticle

Santos, DB, Schiar, VPP, Paixão, MW, Meinerz, DF, Nogueira, CW, Aschner, M, Rocha, JBT & Barbosa, NBV 2009, 'Hemolytic and genotoxic evaluation of organochalcogens in human blood cells in vitro', Toxicology in Vitro, vol. 23, no. 6, pp. 1195-1204. https://doi.org/10.1016/j.tiv.2009.05.010
Santos, D. B. ; Schiar, V. P P ; Paixão, M. W. ; Meinerz, D. F. ; Nogueira, C. W. ; Aschner, Michael ; Rocha, J. B T ; Barbosa, N. B V. / Hemolytic and genotoxic evaluation of organochalcogens in human blood cells in vitro. In: Toxicology in Vitro. 2009 ; Vol. 23, No. 6. pp. 1195-1204.
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