Hemoglobins of two terebellid polychaetes: Enoplobranchus sanguineus and Amphitrite ornata

Roy E. Weber, Charlotte Mangum, Howard M. Steinman, Celia Bonaventura, Bolling Sullivan, Joseph Bonaventura

Research output: Contribution to journalArticle

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Abstract

1. 1. The intracellular, coelomic hemoglobins of the terebellid annelids Enoplobranchus sanguineus and Amphitrite ornata are momoneric proteins possessing high O2 affinities (half-saturation O2 tension, P50 = 1.4 and 2.8 mm, respectively, at 20°C), and lacking homotropic or heterotropic interactions in O2 binding. These O2 affinities are high relative to extracellular vascular hemoglobins, but low relative to mammalian myoglobins, due to considerably higher rates of O2 dissociation. 2. 2. The extracellular, multi-subunit hemoglobin (erythrocruorin) from the vascular system of A. ornata has a low O2 affinity (P50 about 11 mm Hg at 20°C) and shows significant heme-heme interaction (Hill's constant n50 = 1.5-2.0). It lacks a Bohr effect between pH 5.8 and 8.0, but shows a large increase in O2 affinity above pH 8.0 that may be due to dissociation into high affinity subunits. 3. 3. The amino acid composition and the amino acid sequence for the first 29 residues of intracellular E. sanguineus hemoglobin show a surprising lack of homology with other simple heme proteins.

Original languageEnglish (US)
Pages (from-to)179-187
Number of pages9
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume56
Issue number2
DOIs
StatePublished - 1977
Externally publishedYes

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Heme
Blood Vessels
Hemoglobins
Hemoglobin Subunits
Hemoglobin E
Hemeproteins
Myoglobin
pH effects
Amino Acids
Amino Acid Sequence
Proteins
Chemical analysis
erythrocruorin

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Biochemistry

Cite this

Hemoglobins of two terebellid polychaetes : Enoplobranchus sanguineus and Amphitrite ornata. / Weber, Roy E.; Mangum, Charlotte; Steinman, Howard M.; Bonaventura, Celia; Sullivan, Bolling; Bonaventura, Joseph.

In: Comparative Biochemistry and Physiology -- Part A: Physiology, Vol. 56, No. 2, 1977, p. 179-187.

Research output: Contribution to journalArticle

Weber, Roy E. ; Mangum, Charlotte ; Steinman, Howard M. ; Bonaventura, Celia ; Sullivan, Bolling ; Bonaventura, Joseph. / Hemoglobins of two terebellid polychaetes : Enoplobranchus sanguineus and Amphitrite ornata. In: Comparative Biochemistry and Physiology -- Part A: Physiology. 1977 ; Vol. 56, No. 2. pp. 179-187.
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