Hemoglobins from Mycobacterium tuberculosis and Campylobacter jejuni

A Comparative Study with Resonance Raman Spectroscopy

Changyuan Lu, Tsuyoshi Egawa, Masahiro Mukai, Robert K. Poole, Syun-Ru Yeh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Three groups of hemoglobins (Hbs) have been identified in unicellular organisms: (1) the truncated Hbs (trHb) that display a novel two-over-two α-helical structure, (2) the flavohemoglobins (FHb) that comprise a Hb domain with a classical three-over-three α-helical structure and a covalently attached flavin-containing reductase domain, and (3) the single-domain Hbs (sdHb) that exhibit high sequence homology and structural similarity to the Hb domain of FHb. On the basis of phylogenetic analysis, the trHbs can be further divided into three subgroups: TrHb-I, TrHb-II, and TrHb-III. The various classes of Hbs may coexist in the same organism, suggesting distinct functions for each class of Hb. This chapter reviews the structural and functional properties of a TrHb-I (trHbN) and a TrHb-II (trHbO) from Mycobacterium tuberculosis, as well as a TrHb-III (trCtb) and a sdHb (Cgb) from Campylobacter jejuni on the basis of resonance Raman spectroscopic studies.

Original languageEnglish (US)
Pages (from-to)255-286
Number of pages32
JournalMethods in Enzymology
Volume437
DOIs
StatePublished - 2008

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Campylobacter jejuni
Raman Spectrum Analysis
Mycobacterium tuberculosis
Raman spectroscopy
Hemoglobins
Truncated Hemoglobins
Sequence Homology
Oxidoreductases

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hemoglobins from Mycobacterium tuberculosis and Campylobacter jejuni : A Comparative Study with Resonance Raman Spectroscopy. / Lu, Changyuan; Egawa, Tsuyoshi; Mukai, Masahiro; Poole, Robert K.; Yeh, Syun-Ru.

In: Methods in Enzymology, Vol. 437, 2008, p. 255-286.

Research output: Contribution to journalArticle

Lu, Changyuan ; Egawa, Tsuyoshi ; Mukai, Masahiro ; Poole, Robert K. ; Yeh, Syun-Ru. / Hemoglobins from Mycobacterium tuberculosis and Campylobacter jejuni : A Comparative Study with Resonance Raman Spectroscopy. In: Methods in Enzymology. 2008 ; Vol. 437. pp. 255-286.
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