Hemoglobin Miyada: DNA analysis of the anti-Lepore βδ fusion gene

M. C. Driscoll, Y. Ohta, F. Nakamura, A. Bloom, A. Bank

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Abstract

Hemoglobin Miyada, an anti-Lepore hemoglobin, represents the protein product of a nonhomologous crossover between β and δ genes. The mutant globin is β-like from the N terminus to amino acid 12, and δ-like from amino acid 22 through the C terminus, thus predicting a crossover site in the first coding region of the gene. DNA analysis, using multiple restriction endonucleases and hybridization to δ and β globin gene-specific probes, confirms that the βδ hybrid gene 1) is located on a single chromosomal fragment between normal δ and β genes, and 2) has 5' β promoter sequences, δ IVS 1 and 2, and 3' coding and flanking sequences.

Original languageEnglish (US)
Pages (from-to)355-362
Number of pages8
JournalAmerican Journal of Hematology
Volume17
Issue number4
DOIs
Publication statusPublished - 1984
Externally publishedYes

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ASJC Scopus subject areas

  • Hematology

Cite this

Driscoll, M. C., Ohta, Y., Nakamura, F., Bloom, A., & Bank, A. (1984). Hemoglobin Miyada: DNA analysis of the anti-Lepore βδ fusion gene. American Journal of Hematology, 17(4), 355-362. https://doi.org/10.1002/ajh.2830170405