Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves

Iyore A. James, Tai Yi, Shuhei Tara, Cameron A. Best, Alexander J. Stuber, Kejal V. Shah, Blair F. Austin, Tadahisa Sugiura, Yong Ung Lee, Joy Lincoln, Aaron J. Trask, Toshiharu Shinoka, Christopher K. Breuer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Decellularized allograft heart valves have been used as tissue-engineered heart valve (TEHV) scaffolds with promising results; however, little is known about the cellular mechanisms underlying TEHV neotissue formation. To better understand this phenomenon, we developed a murine model of decellularized pulmonary heart valve transplantation using a hemodynamically unloaded heart transplant model. Furthermore, because the hemodynamics of blood flow through a heart valve may influence morphology and subsequent function, we describe a modified loaded heterotopic heart transplant model that led to an increase in blood flow through the pulmonary valve. We report host cell infiltration and endothelialization of implanted decellularized pulmonary valves (dPV) and provide an experimental approach for the study of TEHVs using mouse models.

Original languageEnglish (US)
Pages (from-to)987-994
Number of pages8
JournalTissue Engineering - Part C: Methods
Volume21
Issue number9
DOIs
StatePublished - Sep 1 2015
Externally publishedYes

Fingerprint

Heart Valves
Hemodynamics
Pulmonary Valve
Tissue
Transplants
Lung Transplantation
Blood
Heart Transplantation
Transplantation (surgical)
Allografts
Infiltration
Scaffolds

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves. / James, Iyore A.; Yi, Tai; Tara, Shuhei; Best, Cameron A.; Stuber, Alexander J.; Shah, Kejal V.; Austin, Blair F.; Sugiura, Tadahisa; Lee, Yong Ung; Lincoln, Joy; Trask, Aaron J.; Shinoka, Toshiharu; Breuer, Christopher K.

In: Tissue Engineering - Part C: Methods, Vol. 21, No. 9, 01.09.2015, p. 987-994.

Research output: Contribution to journalArticle

James, IA, Yi, T, Tara, S, Best, CA, Stuber, AJ, Shah, KV, Austin, BF, Sugiura, T, Lee, YU, Lincoln, J, Trask, AJ, Shinoka, T & Breuer, CK 2015, 'Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves', Tissue Engineering - Part C: Methods, vol. 21, no. 9, pp. 987-994. https://doi.org/10.1089/ten.tec.2015.0011
James, Iyore A. ; Yi, Tai ; Tara, Shuhei ; Best, Cameron A. ; Stuber, Alexander J. ; Shah, Kejal V. ; Austin, Blair F. ; Sugiura, Tadahisa ; Lee, Yong Ung ; Lincoln, Joy ; Trask, Aaron J. ; Shinoka, Toshiharu ; Breuer, Christopher K. / Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves. In: Tissue Engineering - Part C: Methods. 2015 ; Vol. 21, No. 9. pp. 987-994.
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