Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves

Iyore A. James, Tai Yi, Shuhei Tara, Cameron A. Best, Alexander J. Stuber, Kejal V. Shah, Blair F. Austin, Tadahisa Sugiura, Yong Ung Lee, Joy Lincoln, Aaron J. Trask, Toshiharu Shinoka, Christopher K. Breuer

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Decellularized allograft heart valves have been used as tissue-engineered heart valve (TEHV) scaffolds with promising results; however, little is known about the cellular mechanisms underlying TEHV neotissue formation. To better understand this phenomenon, we developed a murine model of decellularized pulmonary heart valve transplantation using a hemodynamically unloaded heart transplant model. Furthermore, because the hemodynamics of blood flow through a heart valve may influence morphology and subsequent function, we describe a modified loaded heterotopic heart transplant model that led to an increase in blood flow through the pulmonary valve. We report host cell infiltration and endothelialization of implanted decellularized pulmonary valves (dPV) and provide an experimental approach for the study of TEHVs using mouse models.

Original languageEnglish (US)
Pages (from-to)987-994
Number of pages8
JournalTissue Engineering - Part C: Methods
Volume21
Issue number9
DOIs
Publication statusPublished - Sep 1 2015
Externally publishedYes

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ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

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