Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves

Iyore A. James; Tai Yi; Shuhei Tara; Cameron A. Best; Alexander J. Stuber; Kejal V. Shah; Blair F. Austin; Tadahisa Sugiura; Yong Ung Lee; Joy Lincoln; Aaron J. Trask; Toshiharu Shinoka; Christopher K. Breuer

doi:10.1089/ten.tec.2015.0011

Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves

Iyore A. James, Tai Yi, Shuhei Tara, Cameron A. Best, Alexander J. Stuber, Kejal V. Shah, Blair F. Austin, Tadahisa Sugiura, Yong Ung Lee, Joy Lincoln, Aaron J. Trask, Toshiharu Shinoka, Christopher K. Breuer

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Decellularized allograft heart valves have been used as tissue-engineered heart valve (TEHV) scaffolds with promising results; however, little is known about the cellular mechanisms underlying TEHV neotissue formation. To better understand this phenomenon, we developed a murine model of decellularized pulmonary heart valve transplantation using a hemodynamically unloaded heart transplant model. Furthermore, because the hemodynamics of blood flow through a heart valve may influence morphology and subsequent function, we describe a modified loaded heterotopic heart transplant model that led to an increase in blood flow through the pulmonary valve. We report host cell infiltration and endothelialization of implanted decellularized pulmonary valves (dPV) and provide an experimental approach for the study of TEHVs using mouse models.

Original language	English (US)
Pages (from-to)	987-994
Number of pages	8
Journal	Tissue Engineering - Part C: Methods
Volume	21
Issue number	9
DOIs	https://doi.org/10.1089/ten.tec.2015.0011
State	Published - Sep 1 2015
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Biomedical Engineering

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James, I. A., Yi, T., Tara, S., Best, C. A., Stuber, A. J., Shah, K. V., Austin, B. F., Sugiura, T., Lee, Y. U., Lincoln, J., Trask, A. J., Shinoka, T., & Breuer, C. K. (2015). Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves. Tissue Engineering - Part C: Methods, 21(9), 987-994. https://doi.org/10.1089/ten.tec.2015.0011

James, IA, Yi, T, Tara, S, Best, CA, Stuber, AJ, Shah, KV, Austin, BF, Sugiura, T, Lee, YU, Lincoln, J, Trask, AJ, Shinoka, T & Breuer, CK 2015, 'Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves', Tissue Engineering - Part C: Methods, vol. 21, no. 9, pp. 987-994. https://doi.org/10.1089/ten.tec.2015.0011

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title = "Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves",

abstract = "Decellularized allograft heart valves have been used as tissue-engineered heart valve (TEHV) scaffolds with promising results; however, little is known about the cellular mechanisms underlying TEHV neotissue formation. To better understand this phenomenon, we developed a murine model of decellularized pulmonary heart valve transplantation using a hemodynamically unloaded heart transplant model. Furthermore, because the hemodynamics of blood flow through a heart valve may influence morphology and subsequent function, we describe a modified loaded heterotopic heart transplant model that led to an increase in blood flow through the pulmonary valve. We report host cell infiltration and endothelialization of implanted decellularized pulmonary valves (dPV) and provide an experimental approach for the study of TEHVs using mouse models.",

author = "James, {Iyore A.} and Tai Yi and Shuhei Tara and Best, {Cameron A.} and Stuber, {Alexander J.} and Shah, {Kejal V.} and Austin, {Blair F.} and Tadahisa Sugiura and Lee, {Yong Ung} and Joy Lincoln and Trask, {Aaron J.} and Toshiharu Shinoka and Breuer, {Christopher K.}",

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doi = "10.1089/ten.tec.2015.0011",

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AU - Yi, Tai

AU - Tara, Shuhei

AU - Best, Cameron A.

AU - Stuber, Alexander J.

AU - Shah, Kejal V.

AU - Austin, Blair F.

AU - Sugiura, Tadahisa

AU - Lee, Yong Ung

AU - Lincoln, Joy

AU - Trask, Aaron J.

AU - Shinoka, Toshiharu

AU - Breuer, Christopher K.

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AB - Decellularized allograft heart valves have been used as tissue-engineered heart valve (TEHV) scaffolds with promising results; however, little is known about the cellular mechanisms underlying TEHV neotissue formation. To better understand this phenomenon, we developed a murine model of decellularized pulmonary heart valve transplantation using a hemodynamically unloaded heart transplant model. Furthermore, because the hemodynamics of blood flow through a heart valve may influence morphology and subsequent function, we describe a modified loaded heterotopic heart transplant model that led to an increase in blood flow through the pulmonary valve. We report host cell infiltration and endothelialization of implanted decellularized pulmonary valves (dPV) and provide an experimental approach for the study of TEHVs using mouse models.

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Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves

Abstract

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