Heme-heme interactions in a homodimeric cooperative hemoglobin: Evidence from transient Raman scattering

Denis L. Rousseau, Sunho Song, Joel M. Friedman, Alberto Boffi, Emilia Chiancone

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The comparison of the resonance Raman spectrum of the deoxy dimeric hemoglobin (HbI) from the Arcid clam, Scapharca inaequivalvis, to its CO photoproduct at 10 ns reveals several significant differences in the low frequency vibrational modes including those in-volving motions of the peripheral substituents on the heme such as the propionates. This finding reflects the involvement of the propionates in a hydrogen bonding network which connects the two heme groups across the subunit interface and is sensitive to ligand binding. A frequency difference in the iron-histidine stretching mode of 6 cm-1 in this invertebrate hemoglobin is substantially smaller than that detected in tetrameric vertebrate hemoglobins under similar conditions. Time evolution studies of the vibrational modes from the photoproduct demonstrate that the transient form relaxes to the deoxy structure concertedly with a halflife of 1 its, the time scale in which tertiary relaxations of tetrameric hemoglobins occur. These data establish that the tertiary and quaternary structural changes take place on the same time scale and confirm a mechanism of cooperativity involving direct interaction between the two heme groups through the peripheral substituents. This direct structural communication provides a very tight linkage between the heme groups prohibiting modulation of the oxygen affinity by factors in the physiological milieu.

Original languageEnglish (US)
Pages (from-to)5719-5723
Number of pages5
JournalJournal of Biological Chemistry
Volume268
Issue number8
StatePublished - Mar 15 1993
Externally publishedYes

Fingerprint

Raman Spectrum Analysis
Heme
Raman scattering
Hemoglobins
Propionates
Scapharca
Bivalvia
Vibrational spectra
Invertebrates
Hydrogen Bonding
Carbon Monoxide
Histidine
Stretching
Half-Life
Vertebrates
Hydrogen bonds
Iron
Modulation
Oxygen
Ligands

ASJC Scopus subject areas

  • Biochemistry

Cite this

Heme-heme interactions in a homodimeric cooperative hemoglobin : Evidence from transient Raman scattering. / Rousseau, Denis L.; Song, Sunho; Friedman, Joel M.; Boffi, Alberto; Chiancone, Emilia.

In: Journal of Biological Chemistry, Vol. 268, No. 8, 15.03.1993, p. 5719-5723.

Research output: Contribution to journalArticle

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