Heme distortion modulated by ligand-protein interactions in inducible nitric-oxide synthase

David Lis, Dennis J. Stuehr, Syun-Ru Yeh, Denis L. Rousseau

Research output: Contribution to journalArticle

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Abstract

The catalytic center of nitric-oxide synthase (NOS) consists of a thiolate-coordinated heme macrocycle, a tetrahydrobiopterin (H4B) cofactor, and an L-arginine (L-Arg)/N-hydroxyarginine substrate binding site. To determine how the interplay between the cofactor, the substrates, and the protein matrix housing the heme regulates the enzymatic activity of NOS, the CO-, NO-, and CN-bound adducts of the oxygenase domain of the inducible isoform of NOS (iNOSoxy) were examined with resonance Raman spectroscopy. The Raman data of the CO-bound ferrous protein demonstrated that the presence of L-Arg causes the Fe-C-O moiety to adopt a bent structure because of an H-bonding interaction whereas H4B binding exerts no effect. Similar behavior was found in the CN-bound ferric protein and in the nitric oxide (NO)-bound ferrous protein. In contrast, in the NO-bound ferric complexes, the addition of L-Arg alone does not affect the structural properties of the Fe-N-O moiety, but H4B binding forces it to adopt a bent structure, which is further enhanced by the subsequent addition of L-Arg. The differential interactions between the various heme ligands and the protein matrix in response to L-Arg and/or H4B binding is coupled to heme distortions, as reflected by the development of a variety of out-of-plane heme modes in the low frequency Raman spectra. The extent and symmetry of heme deformation modulated by ligand, substrate, and cofactor binding may provide important control over the catalytic and autoinhibitory properties of the enzyme.

Original languageEnglish (US)
Pages (from-to)26489-26499
Number of pages11
JournalJournal of Biological Chemistry
Volume279
Issue number25
DOIs
StatePublished - Jun 18 2004

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Nitric Oxide Synthase Type II
Heme
Ligands
Nitric Oxide Synthase
Nitric Oxide
Proteins
Carbon Monoxide
Substrates
Oxygenases
Raman Spectrum Analysis
Arginine
Raman spectroscopy
Raman scattering
Structural properties
Protein Isoforms
Binding Sites
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Heme distortion modulated by ligand-protein interactions in inducible nitric-oxide synthase. / Lis, David; Stuehr, Dennis J.; Yeh, Syun-Ru; Rousseau, Denis L.

In: Journal of Biological Chemistry, Vol. 279, No. 25, 18.06.2004, p. 26489-26499.

Research output: Contribution to journalArticle

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