Hematopoietic progenitor cell rolling in bone marrow microvessels: Parallel contributions by endothelial selectins and vascular cell adhesion molecule 1

Irina B. Mazo, Jose Carlos Gutierrez-Ramos, Paul S. Frenette, Richard O. Hynes, Denisa D. Wagner, Ulrich H. Von Andrian

Research output: Contribution to journalArticle

343 Scopus citations

Abstract

We have used intravital microscopy to study physiologically perfused microvessels in murine bone marrow (BM). BM sinusoids and venules, but not adjacent bone vessels, supported rolling interactions of hematopoietic progenitor cells. Rolling did not involve L-selectin, but was partially reduced in wild-type mice treated with antibodies to P- or E-selectin and in mice that were deficient in these two selectins. Selectin-independent rolling was mediated by α4 integrins, which interacted with endothelial vascular cell adhesion molecule (VCAM)-1. Parallel contribution of the endothelial selectins and VCAM-1 is not known to direct blood cell trafficking to other noninflamed tissues. This combination of constitutively expressed adhesion molecules may thus constitute a BM-specific recruitment pathway for progenitor cells analogous to the vascular addressins that direct selective lymphocyte homing to lymphoid organs.

Original languageEnglish (US)
Pages (from-to)465-474
Number of pages10
JournalJournal of Experimental Medicine
Volume188
Issue number3
DOIs
StatePublished - Aug 3 1998

Keywords

  • Bone marrow
  • Homing
  • Intravital microscopy
  • Selectins
  • α4 integrin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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