Helical structure and folding of subunit c of F1Fo ATP synthase: 1H NMR resonance assignments and NOE analysis

Mark E. Girvin, Robert H. Fillingame

Research output: Contribution to journalArticle

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Abstract

Subunit c of the H+-transporting F1Fo ATP synthase (EC 3.6.1.34) is thought to fold across the membrane as a hairpin of two α-helices and function as a key component of the H+-translocase of Fo. We report here the initial results of a structural study of purified subunit c in a chloroform-methanol-water (4:4:1) solvent mixture using standard two-dimensional NMR techniques. The spin systems of 78 of the 79 amino acid side chains have been assigned to residue type, and 44 of these have been assigned to specific residues in the sequence. Stretches of α-helical secondary structure were observed for Asp7-Ile26 in the first proposed transmembrane helix, and for Arg50-Ile55 and Ala67-Val78 in the second proposed transmembrane helix. Nuclear Overhauser effects (NOEs) were observed between residues at both ends of the predicted transmembrane helices. The intensities of the NOEs between helix-1 and helix-2 were not diminished by mixing of 2H-subunit c with 1H-subunit c, and therefore the NOEs must be due to intramolecular, rather than intermolecular, interactions. Hence the purified protein must fold as a hairpin in this solvent system, just as it is thought to fold in the lipid bilayer of the membrane. In native Fo, dicyclohexylcarbodiimide reacts specifically with Asp61 in the second transmembrane helix of subunit c, and the rate of this reaction is reduced by substitution of Ile28 by Thr on the first transmembrane helix. The I28T substitution is shown here to alter the chemical shifts of protons at and around Asp61. This observation provides a further indication that subunit c may fold in chloroform-methanol-water solvent much like it does in the membrane.

Original languageEnglish (US)
Pages (from-to)12167-12177
Number of pages11
JournalBiochemistry
Volume32
Issue number45
StatePublished - 1993
Externally publishedYes

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Adenosine Triphosphate
Nuclear magnetic resonance
Chloroform
Membranes
Methanol
Substitution reactions
Dicyclohexylcarbodiimide
Lipid bilayers
Proton-Translocating ATPases
Water
Chemical shift
Lipid Bilayers
Membrane Lipids
Protons
Amino Acids
Proton Magnetic Resonance Spectroscopy
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Helical structure and folding of subunit c of F1Fo ATP synthase : 1H NMR resonance assignments and NOE analysis. / Girvin, Mark E.; Fillingame, Robert H.

In: Biochemistry, Vol. 32, No. 45, 1993, p. 12167-12177.

Research output: Contribution to journalArticle

Girvin, Mark E. ; Fillingame, Robert H. / Helical structure and folding of subunit c of F1Fo ATP synthase : 1H NMR resonance assignments and NOE analysis. In: Biochemistry. 1993 ; Vol. 32, No. 45. pp. 12167-12177.
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abstract = "Subunit c of the H+-transporting F1Fo ATP synthase (EC 3.6.1.34) is thought to fold across the membrane as a hairpin of two α-helices and function as a key component of the H+-translocase of Fo. We report here the initial results of a structural study of purified subunit c in a chloroform-methanol-water (4:4:1) solvent mixture using standard two-dimensional NMR techniques. The spin systems of 78 of the 79 amino acid side chains have been assigned to residue type, and 44 of these have been assigned to specific residues in the sequence. Stretches of α-helical secondary structure were observed for Asp7-Ile26 in the first proposed transmembrane helix, and for Arg50-Ile55 and Ala67-Val78 in the second proposed transmembrane helix. Nuclear Overhauser effects (NOEs) were observed between residues at both ends of the predicted transmembrane helices. The intensities of the NOEs between helix-1 and helix-2 were not diminished by mixing of 2H-subunit c with 1H-subunit c, and therefore the NOEs must be due to intramolecular, rather than intermolecular, interactions. Hence the purified protein must fold as a hairpin in this solvent system, just as it is thought to fold in the lipid bilayer of the membrane. In native Fo, dicyclohexylcarbodiimide reacts specifically with Asp61 in the second transmembrane helix of subunit c, and the rate of this reaction is reduced by substitution of Ile28 by Thr on the first transmembrane helix. The I28T substitution is shown here to alter the chemical shifts of protons at and around Asp61. This observation provides a further indication that subunit c may fold in chloroform-methanol-water solvent much like it does in the membrane.",
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