Heavy chain producing variants of a mouse myeloma cell line

S. L. Morrison, M. D. Scharff

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Eleven variants producing only heavy (H) chains have been isolated from the P3 cell line after mutagenesis with either ICR 191 or nitrosoguanidine. The H chains produced by these variants are similar both to molecular weight and by peptide maps with the parental H chain. H chains dimerize intracellularly, are not secreted but persist as stable cytoplasmic proteins. No evidence for the continued synthesis of light (L) chains or L chain fragments has been found in these variants. These studies show that, in the P3 cell line, the synthesis of H chains continue in the absence of detectable L chain synthesis.

Original languageEnglish (US)
Pages (from-to)655-659
Number of pages5
JournalJournal of Immunology
Volume114
Issue number2 I
StatePublished - Dec 1 1975

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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