Heavy chain producing variants of a mouse myeloma cell line

S. L. Morrison, M. D. Scharff

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Abstract

Eleven variants producing only heavy (H) chains have been isolated from the P3 cell line after mutagenesis with either ICR 191 or nitrosoguanidine. The H chains produced by these variants are similar both to molecular weight and by peptide maps with the parental H chain. H chains dimerize intracellularly, are not secreted but persist as stable cytoplasmic proteins. No evidence for the continued synthesis of light (L) chains or L chain fragments has been found in these variants. These studies show that, in the P3 cell line, the synthesis of H chains continue in the absence of detectable L chain synthesis.

Original languageEnglish (US)
Pages (from-to)655-659
Number of pages5
JournalJournal of Immunology
Volume114
Issue number2 I
Publication statusPublished - Dec 1 1975

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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