Heat stress of cultured GC cells enhances triiodothyronine-induced growth hormone production by action within the 5′- flanking region of the rat growth hormone gene

Sri Prakash Mokshagundam; Lawrence E. Shapiro; Martin I. Surks

doi:10.1016/0006-291X(92)91104-X

Heat stress of cultured GC cells enhances triiodothyronine-induced growth hormone production by action within the 5′- flanking region of the rat growth hormone gene

Sri Prakash Mokshagundam, Lawrence E. Shapiro, Martin I. Surks

Medicine

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Abstract

We studied the effect of incubation at 41 C on a clone of GC cells that had previously been stably transfected with a gene construct, pGHXGPT, containing -1800 to +8 of the rat growth hormone promoter fused to the structural gene for E. Coli xanthine guanine phosphor ibosyl-transferase. The effect of incubation of the clone containing pGHXGPT at 41 C was to enhance triiodothyronine induction of growth hormone secretion (2-fold, p<0.01) and of xanthine quanine phosphoribosyl-transferase activity (3-fold, p< 0.01). We conclude that the increase in triiodothyronine-induced growth hormone production during heat stress occurs by stimulation of the growth hormone promoter.

Original language	English (US)
Pages (from-to)	638-643
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	188
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(92)91104-X
State	Published - Oct 30 1992

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Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Heat stress of cultured GC cells enhances triiodothyronine-induced growth hormone production by action within the 5′- flanking region of the rat growth hormone gene. / Mokshagundam, Sri Prakash; Shapiro, Lawrence E.; Surks, Martin I.
In: Biochemical and Biophysical Research Communications, Vol. 188, No. 2, 30.10.1992, p. 638-643.

Research output: Contribution to journal › Article › peer-review

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title = "Heat stress of cultured GC cells enhances triiodothyronine-induced growth hormone production by action within the 5′- flanking region of the rat growth hormone gene",

abstract = "We studied the effect of incubation at 41 C on a clone of GC cells that had previously been stably transfected with a gene construct, pGHXGPT, containing -1800 to +8 of the rat growth hormone promoter fused to the structural gene for E. Coli xanthine guanine phosphor ibosyl-transferase. The effect of incubation of the clone containing pGHXGPT at 41 C was to enhance triiodothyronine induction of growth hormone secretion (2-fold, p<0.01) and of xanthine quanine phosphoribosyl-transferase activity (3-fold, p< 0.01). We conclude that the increase in triiodothyronine-induced growth hormone production during heat stress occurs by stimulation of the growth hormone promoter.",

author = "Mokshagundam, {Sri Prakash} and Shapiro, {Lawrence E.} and Surks, {Martin I.}",

note = "Funding Information: Herbert H. Samuels for providing the Christine Frawley and Evette Gonzalez for This work was supported by NIH Grants and NIH Training Grant AM 07004 (to S.M.)",

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AU - Shapiro, Lawrence E.

AU - Surks, Martin I.

N1 - Funding Information: Herbert H. Samuels for providing the Christine Frawley and Evette Gonzalez for This work was supported by NIH Grants and NIH Training Grant AM 07004 (to S.M.)

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N2 - We studied the effect of incubation at 41 C on a clone of GC cells that had previously been stably transfected with a gene construct, pGHXGPT, containing -1800 to +8 of the rat growth hormone promoter fused to the structural gene for E. Coli xanthine guanine phosphor ibosyl-transferase. The effect of incubation of the clone containing pGHXGPT at 41 C was to enhance triiodothyronine induction of growth hormone secretion (2-fold, p<0.01) and of xanthine quanine phosphoribosyl-transferase activity (3-fold, p< 0.01). We conclude that the increase in triiodothyronine-induced growth hormone production during heat stress occurs by stimulation of the growth hormone promoter.

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Heat stress of cultured GC cells enhances triiodothyronine-induced growth hormone production by action within the 5′- flanking region of the rat growth hormone gene

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