Heat stress of cultured GC cells enhances triiodothyronine-induced growth hormone production by action within the 5′- flanking region of the rat growth hormone gene

Sri Prakash Mokshagundam, Lawrence E. Shapiro, Martin I. Surks

Research output: Contribution to journalArticle

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Abstract

We studied the effect of incubation at 41 C on a clone of GC cells that had previously been stably transfected with a gene construct, pGHXGPT, containing -1800 to +8 of the rat growth hormone promoter fused to the structural gene for E. Coli xanthine guanine phosphor ibosyl-transferase. The effect of incubation of the clone containing pGHXGPT at 41 C was to enhance triiodothyronine induction of growth hormone secretion (2-fold, p<0.01) and of xanthine quanine phosphoribosyl-transferase activity (3-fold, p< 0.01). We conclude that the increase in triiodothyronine-induced growth hormone production during heat stress occurs by stimulation of the growth hormone promoter.

Original languageEnglish (US)
Pages (from-to)638-643
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume188
Issue number2
DOIs
Publication statusPublished - Oct 30 1992

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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