Heat stress of cultured GC cells enhances triiodothyronine-induced growth hormone production by action within the 5′- flanking region of the rat growth hormone gene

SriPrakash Mokshagundam, Lawrence E. Shapiro, Martin I. Surks

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We studied the effect of incubation at 41 C on a clone of GC cells that had previously been stably transfected with a gene construct, pGHXGPT, containing -1800 to +8 of the rat growth hormone promoter fused to the structural gene for E. Coli xanthine guanine phosphor ibosyl-transferase. The effect of incubation of the clone containing pGHXGPT at 41 C was to enhance triiodothyronine induction of growth hormone secretion (2-fold, p<0.01) and of xanthine quanine phosphoribosyl-transferase activity (3-fold, p< 0.01). We conclude that the increase in triiodothyronine-induced growth hormone production during heat stress occurs by stimulation of the growth hormone promoter.

Original languageEnglish (US)
Pages (from-to)638-643
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume188
Issue number2
DOIs
StatePublished - Oct 30 1992

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5' Flanking Region
Triiodothyronine
Growth Hormone
Rats
Cultured Cells
Hot Temperature
Genes
Xanthine
Transferases
Clone Cells
Thermogenesis
Guanine
Phosphors
Escherichia coli

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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abstract = "We studied the effect of incubation at 41 C on a clone of GC cells that had previously been stably transfected with a gene construct, pGHXGPT, containing -1800 to +8 of the rat growth hormone promoter fused to the structural gene for E. Coli xanthine guanine phosphor ibosyl-transferase. The effect of incubation of the clone containing pGHXGPT at 41 C was to enhance triiodothyronine induction of growth hormone secretion (2-fold, p<0.01) and of xanthine quanine phosphoribosyl-transferase activity (3-fold, p< 0.01). We conclude that the increase in triiodothyronine-induced growth hormone production during heat stress occurs by stimulation of the growth hormone promoter.",
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T1 - Heat stress of cultured GC cells enhances triiodothyronine-induced growth hormone production by action within the 5′- flanking region of the rat growth hormone gene

AU - Mokshagundam, SriPrakash

AU - Shapiro, Lawrence E.

AU - Surks, Martin I.

PY - 1992/10/30

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