Guanosine and synthetic organoselenium compounds modulate methylmercury-induced oxidative stress in rat brain cortical slices: Involvement of oxidative stress and glutamatergic system

Daniel H. Roos, Robson L. Puntel, Matheus M. Santos, Diogo O G Souza, Marcelo Farina, Cristina W. Nogueira, Michael Aschner, Marilise Escobar Burger, Nilda B V Barbosa, João B T Rocha

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Excessive formation of reactive oxygen species (ROS) and disruption of glutamate uptake have been pointed as two key mechanisms in methylmercury-toxicity. Thus, here we investigate the involvement of glutamatergic system in methylmercury (MeHg) neurotoxicity and whether diphenyl diselenide, ebselen and guanosine could protect cortical rat brain slices from MeHg-induced ROS generation. MeHg (100 and 200 μM) increased 2′,7′-dichlorodihydrofluorescin (DCFH) oxidation after 2 h of exposure. At 50 μM, MeHg increased DCFH oxidation only after 5 h of exposure. Guanosine (1 and 5 μM) did not caused any effect per se; however, it blocked the increase in DCFH caused by 200 or 50 μM MeHg. Ebselen (5 and 10 μM) decreased significantly the DCFH oxidation after 2 and 5 h of exposure to MeHg. Diphenyl diselenide (5 μM) did not change the basal DCFH oxidation, but abolished the pro-oxidant effect of MeHg. MK-801 also abolished the pro-oxidant effect of MeHg. These results demonstrate for the first time the potential antioxidant properties of organoseleniun compounds and guanosine against MeHg-induced ROS generation after short-term exposure in a simple in vitro model. In conclusion, endogenous purine (guanosine) and two synthetic organoselenium compounds can modulate the pro-oxidant effect of MeHg in cortical brain slices.

Original languageEnglish (US)
Pages (from-to)302-307
Number of pages6
JournalToxicology in Vitro
Volume23
Issue number2
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Organoselenium Compounds
Oxidative stress
Guanosine
Rats
Reactive Oxygen Species
Brain
Oxidative Stress
Oxidation
Dizocilpine Maleate
Toxicity
Glutamic Acid
Antioxidants

Keywords

  • Free radicals
  • Glutamatergic system
  • MeHg

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology

Cite this

Guanosine and synthetic organoselenium compounds modulate methylmercury-induced oxidative stress in rat brain cortical slices : Involvement of oxidative stress and glutamatergic system. / Roos, Daniel H.; Puntel, Robson L.; Santos, Matheus M.; Souza, Diogo O G; Farina, Marcelo; Nogueira, Cristina W.; Aschner, Michael; Burger, Marilise Escobar; Barbosa, Nilda B V; Rocha, João B T.

In: Toxicology in Vitro, Vol. 23, No. 2, 2009, p. 302-307.

Research output: Contribution to journalArticle

Roos, Daniel H. ; Puntel, Robson L. ; Santos, Matheus M. ; Souza, Diogo O G ; Farina, Marcelo ; Nogueira, Cristina W. ; Aschner, Michael ; Burger, Marilise Escobar ; Barbosa, Nilda B V ; Rocha, João B T. / Guanosine and synthetic organoselenium compounds modulate methylmercury-induced oxidative stress in rat brain cortical slices : Involvement of oxidative stress and glutamatergic system. In: Toxicology in Vitro. 2009 ; Vol. 23, No. 2. pp. 302-307.
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