Growth of murine sarcoma virus transformed rat kidney cells in nude mice: absence of induction of host endogenous viruses

J. A. Bilello, Victoria H. Freedman, S. Shin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Clonal isolates of the normal rat kidney cell line (NRK) transformed by a defective murine sarcoma virus (Kirsten strain) were injected into nude mice of BALB/c background to determine whether the growth of these cells as tumors was accompanied by the induction of host endogenous type C viruses. All the virus transformed clones produced rapidly growing tumors in nude mice, but neither the induction of mouse endogenous viruses nor the rescue and spread of the transforming sarcoma virus were observed during the growth of tumors. The degree of expression of the tumor virus structural proteins in the transformed cells did not determine the cellular phenotype with regard to tumorigenicity in nude mice, nor did it modify the cellular growth properties in vitro. Consistent with earlier observations with simian virus 40 transformed mouse and rat cells, the ability of sarcoma virus transformed NRK cells to initiate tumor growth in nude mice appeared to be correlated with anchorage independent growth in vitro.

Original languageEnglish (US)
Pages (from-to)1691-1694
Number of pages4
JournalJournal of the National Cancer Institute
Volume58
Issue number6
StatePublished - 1977

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Murine Sarcoma Viruses
Nude Mice
Viruses
Kidney
Growth
Sarcoma
Neoplasms
Viral Structural Proteins
Defective Viruses
Oncogenic Viruses
Transformed Cell Line
Simian virus 40
Clone Cells
Phenotype

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

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abstract = "Clonal isolates of the normal rat kidney cell line (NRK) transformed by a defective murine sarcoma virus (Kirsten strain) were injected into nude mice of BALB/c background to determine whether the growth of these cells as tumors was accompanied by the induction of host endogenous type C viruses. All the virus transformed clones produced rapidly growing tumors in nude mice, but neither the induction of mouse endogenous viruses nor the rescue and spread of the transforming sarcoma virus were observed during the growth of tumors. The degree of expression of the tumor virus structural proteins in the transformed cells did not determine the cellular phenotype with regard to tumorigenicity in nude mice, nor did it modify the cellular growth properties in vitro. Consistent with earlier observations with simian virus 40 transformed mouse and rat cells, the ability of sarcoma virus transformed NRK cells to initiate tumor growth in nude mice appeared to be correlated with anchorage independent growth in vitro.",
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AU - Shin, S.

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