Growth hormone stimulates skeletal muscle protein synthesis and antagonizes insulin's antiproteolytic action in humans

David A. Fryburg, Rita Jean Louard, Karynn E. Gerow, Robert A. Gelfand, Eugene J. Barrett

Research output: Contribution to journalArticle

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Abstract

We examined the effects of a combined, local intra-arterial infusion of growth hormone (GH) and insulin on forearm glucose and protein metabolism in seven normal adults. GH was infused into the brachial artery for 6 h with a dose that, in a previous study, stimulated muscle protein synthesis (phenylalanine Rd) without affecting systemic GH, insulin, or insulinlike growth factor I concentrations. For the last 3 h of the GH infusion, insulin was coinfused with a dose that, in the absence of infused GH, suppressed forearm muscle proteolysis by 30-40% without affecting systemic insulin levels. Measurements of forearm glucose, amino acid balance, and [3H]phenylalanine and [14C]leucine kinetics were made at 3 and 6 h of the infusion. Glucose uptake by forearm tissues in response to GH and insulin did not change significantly between 3 and 6 h. By 6 h, the combined infusion of GH and insulin promoted a significantly more positive net balance of phenylalanine, leucine, isoleucine, and valine (all P < 0.05). The change in net phenylalanine balance was due to a significant increase in phenylalanine Rd (51%, P < 0.05) with no observable change in phenylalanine Ra. For leucine, a stimulation of leucine Rd (50%, P < 0.05) also accounted for the change in leucine net balance, with no suppression of leucine Ra. The stimulation of Rd, in the absence of an observed effect on Ra, suggests that GH blunts the action of insulin to suppress proteolysis in addition to blunting insulin's action on Rd.

Original languageEnglish (US)
Pages (from-to)424-429
Number of pages6
JournalDiabetes
Volume41
Issue number4
StatePublished - Apr 1992
Externally publishedYes

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Muscle Proteins
Growth Hormone
Skeletal Muscle
Phenylalanine
Leucine
Insulin
Forearm
Glucose
Proteolysis
Intra Arterial Infusions
Brachial Artery
Isoleucine
Valine
Intercellular Signaling Peptides and Proteins
Amino Acids
Muscles

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Growth hormone stimulates skeletal muscle protein synthesis and antagonizes insulin's antiproteolytic action in humans. / Fryburg, David A.; Louard, Rita Jean; Gerow, Karynn E.; Gelfand, Robert A.; Barrett, Eugene J.

In: Diabetes, Vol. 41, No. 4, 04.1992, p. 424-429.

Research output: Contribution to journalArticle

Fryburg, David A. ; Louard, Rita Jean ; Gerow, Karynn E. ; Gelfand, Robert A. ; Barrett, Eugene J. / Growth hormone stimulates skeletal muscle protein synthesis and antagonizes insulin's antiproteolytic action in humans. In: Diabetes. 1992 ; Vol. 41, No. 4. pp. 424-429.
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