Growth and stability of a cholesterol-independent Semliki Forest virus mutant in mosquitoes

Anna Ahn, Randal J. Schoepp, David Sternberg, Margaret Kielian

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Semliki Forest virus (SFV) is an enveloped alphavirus that is transmitted in the wild by mosquito vectors. In tissue culture cells, SFV requires cholesterol in the cell membrane both for virus membrane fusion and for the efficient exit of progeny virus from the cell. A previously isolated SFV mutant, srf-3, is strikingly less cholesterol-dependent for virus fusion, exit, and growth due to a single amine acid change in the E1 spike protein subunit, proline 226 to serine. Here we show that when mosquitoes were infected by intrathoracic injection at a range of virus multiplicities, the growth of srf-3 was significantly more rapid than that of wild-type virus, particularly at low multiplicity infection. The differential cholesterol requirements for wild-type and srf-3 infection were maintained during virus passage through mosquitoes. The presence or absence of cholesterol in the srf-3 virus membrane did not affect its infection properties in mosquitoes. Thus the srf-3 mutation causes a growth advantage in the tissues of the mosquito host.

Original languageEnglish (US)
Pages (from-to)452-456
Number of pages5
JournalVirology
Volume262
Issue number2
DOIs
StatePublished - Sep 30 1999

Fingerprint

Semliki forest virus
Culicidae
Cholesterol
Viruses
Growth
Infection
Alphavirus
Virus Internalization
Protein Subunits
Proline
Serine
Amines
Cell Culture Techniques
Cell Membrane
Mutation
Injections
Acids
Membranes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Growth and stability of a cholesterol-independent Semliki Forest virus mutant in mosquitoes. / Ahn, Anna; Schoepp, Randal J.; Sternberg, David; Kielian, Margaret.

In: Virology, Vol. 262, No. 2, 30.09.1999, p. 452-456.

Research output: Contribution to journalArticle

Ahn, Anna ; Schoepp, Randal J. ; Sternberg, David ; Kielian, Margaret. / Growth and stability of a cholesterol-independent Semliki Forest virus mutant in mosquitoes. In: Virology. 1999 ; Vol. 262, No. 2. pp. 452-456.
@article{52e5933455ff4378a083012a6708ba68,
title = "Growth and stability of a cholesterol-independent Semliki Forest virus mutant in mosquitoes",
abstract = "Semliki Forest virus (SFV) is an enveloped alphavirus that is transmitted in the wild by mosquito vectors. In tissue culture cells, SFV requires cholesterol in the cell membrane both for virus membrane fusion and for the efficient exit of progeny virus from the cell. A previously isolated SFV mutant, srf-3, is strikingly less cholesterol-dependent for virus fusion, exit, and growth due to a single amine acid change in the E1 spike protein subunit, proline 226 to serine. Here we show that when mosquitoes were infected by intrathoracic injection at a range of virus multiplicities, the growth of srf-3 was significantly more rapid than that of wild-type virus, particularly at low multiplicity infection. The differential cholesterol requirements for wild-type and srf-3 infection were maintained during virus passage through mosquitoes. The presence or absence of cholesterol in the srf-3 virus membrane did not affect its infection properties in mosquitoes. Thus the srf-3 mutation causes a growth advantage in the tissues of the mosquito host.",
author = "Anna Ahn and Schoepp, {Randal J.} and David Sternberg and Margaret Kielian",
year = "1999",
month = "9",
day = "30",
doi = "10.1006/viro.1999.9932",
language = "English (US)",
volume = "262",
pages = "452--456",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Growth and stability of a cholesterol-independent Semliki Forest virus mutant in mosquitoes

AU - Ahn, Anna

AU - Schoepp, Randal J.

AU - Sternberg, David

AU - Kielian, Margaret

PY - 1999/9/30

Y1 - 1999/9/30

N2 - Semliki Forest virus (SFV) is an enveloped alphavirus that is transmitted in the wild by mosquito vectors. In tissue culture cells, SFV requires cholesterol in the cell membrane both for virus membrane fusion and for the efficient exit of progeny virus from the cell. A previously isolated SFV mutant, srf-3, is strikingly less cholesterol-dependent for virus fusion, exit, and growth due to a single amine acid change in the E1 spike protein subunit, proline 226 to serine. Here we show that when mosquitoes were infected by intrathoracic injection at a range of virus multiplicities, the growth of srf-3 was significantly more rapid than that of wild-type virus, particularly at low multiplicity infection. The differential cholesterol requirements for wild-type and srf-3 infection were maintained during virus passage through mosquitoes. The presence or absence of cholesterol in the srf-3 virus membrane did not affect its infection properties in mosquitoes. Thus the srf-3 mutation causes a growth advantage in the tissues of the mosquito host.

AB - Semliki Forest virus (SFV) is an enveloped alphavirus that is transmitted in the wild by mosquito vectors. In tissue culture cells, SFV requires cholesterol in the cell membrane both for virus membrane fusion and for the efficient exit of progeny virus from the cell. A previously isolated SFV mutant, srf-3, is strikingly less cholesterol-dependent for virus fusion, exit, and growth due to a single amine acid change in the E1 spike protein subunit, proline 226 to serine. Here we show that when mosquitoes were infected by intrathoracic injection at a range of virus multiplicities, the growth of srf-3 was significantly more rapid than that of wild-type virus, particularly at low multiplicity infection. The differential cholesterol requirements for wild-type and srf-3 infection were maintained during virus passage through mosquitoes. The presence or absence of cholesterol in the srf-3 virus membrane did not affect its infection properties in mosquitoes. Thus the srf-3 mutation causes a growth advantage in the tissues of the mosquito host.

UR - http://www.scopus.com/inward/record.url?scp=0033619166&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033619166&partnerID=8YFLogxK

U2 - 10.1006/viro.1999.9932

DO - 10.1006/viro.1999.9932

M3 - Article

VL - 262

SP - 452

EP - 456

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -