Growth and muscle defects in mice lacking adult myosin heavy chain genes

Leslie J.R. Acakpo-Satchivi, Winfried Edelmann, Carol Sartorius, Brian D. Lu, Philip A. Wahr, Simon C. Watkins, Joseph M. Metzger, Leslie Leinwand, Raju Kucherlapati

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

The three adult fast myosin heavy chains (MyHCs) constitute the vast majority of the myosin in adult skeletal musculature, and are >92% identical. We describe mice carrying null mutations in each of two predominant adult fast MyHC genes, lib and IId/x. Both null strains exhibit growth and muscle defects, but the defects are different between the two strains and do not correlate with the abundance or distribution of each gene product. For example, despite the fact that MyHC-IIb accounts for >70% of the myosin in skeletal muscle and shows the broadest distribution of expression, the phenotypes of IIb null mutants are generally milder than in the MyHC-IId/x null strain. In addition, in a muscle which expresses both lib and IId/x MyHC in wild-type mice, the histological defects are completely different for null expression of the two genes. Most striking is that while both null strains exhibit physiological defects in isolated muscles, the defects are distinct. Muscle from IIb null mice has significantly reduced ability to generate force while IId null mouse muscle generates normal amounts of force, but has altered kinetic properties. Many of the phenotypes demonstrated by these mice are typical in human muscle disease and should provide insight into their etiology.

Original languageEnglish (US)
Pages (from-to)1219-1229
Number of pages11
JournalJournal of Cell Biology
Volume139
Issue number5
DOIs
StatePublished - Dec 1 1997

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Growth and muscle defects in mice lacking adult myosin heavy chain genes'. Together they form a unique fingerprint.

  • Cite this

    Acakpo-Satchivi, L. J. R., Edelmann, W., Sartorius, C., Lu, B. D., Wahr, P. A., Watkins, S. C., Metzger, J. M., Leinwand, L., & Kucherlapati, R. (1997). Growth and muscle defects in mice lacking adult myosin heavy chain genes. Journal of Cell Biology, 139(5), 1219-1229. https://doi.org/10.1083/jcb.139.5.1219