Growth and morphological transformations of Helicobacter pylori in broth media

Anders P. Andersen, David A. Elliott, Moira Lawson, Peter Barland, Victor Bernard Hatcher, Elena G. Puszkin

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Helicobacter pylori, a cause of peptic ulcer disease and certain types of gastric cancers, has usually been cultured on diverse agar-based media, resulting in a requirement for 2 to 4 days of growth at 37°C. We have developed a novel broth medium consisting of a base medium supplemented with 2% newborn calf serum, Mg2+, Cu2+, Fe2+, Zn2+, Mn2+, and 1 mg of lysed human erythrocytes per ml. This medium supports rapid growth of H. pylori, with a doubling time of about 50 min. Optimal growth was obtained in a pH range higher than that supporting most other gram-negative bacteria (at pH 8.5). H. pylori cultured in this supplemented broth retains the spiral morphology seen in both histological sections and cultures from agar-based media and also retains a high urease activity. After 18 h in this broth, H. pylori transforms to a coccal form with a complete loss of urease activity. Previously these cocci have been reported to be senescent, since they could not be subcultured on agar medium. Our experiments suggest that some of the cocci can revert back to the spiral morphology with full recovery of urease activity when subcultured in fresh microaerobic broth medium.

Original languageEnglish (US)
Pages (from-to)2918-2922
Number of pages5
JournalJournal of Clinical Microbiology
Volume35
Issue number11
StatePublished - Nov 1997

Fingerprint

Helicobacter pylori
Urease
Agar
Growth
Gram-Negative Bacteria
Peptic Ulcer
Stomach Neoplasms
Erythrocytes
Serum

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Andersen, A. P., Elliott, D. A., Lawson, M., Barland, P., Hatcher, V. B., & Puszkin, E. G. (1997). Growth and morphological transformations of Helicobacter pylori in broth media. Journal of Clinical Microbiology, 35(11), 2918-2922.

Growth and morphological transformations of Helicobacter pylori in broth media. / Andersen, Anders P.; Elliott, David A.; Lawson, Moira; Barland, Peter; Hatcher, Victor Bernard; Puszkin, Elena G.

In: Journal of Clinical Microbiology, Vol. 35, No. 11, 11.1997, p. 2918-2922.

Research output: Contribution to journalArticle

Andersen, AP, Elliott, DA, Lawson, M, Barland, P, Hatcher, VB & Puszkin, EG 1997, 'Growth and morphological transformations of Helicobacter pylori in broth media', Journal of Clinical Microbiology, vol. 35, no. 11, pp. 2918-2922.
Andersen AP, Elliott DA, Lawson M, Barland P, Hatcher VB, Puszkin EG. Growth and morphological transformations of Helicobacter pylori in broth media. Journal of Clinical Microbiology. 1997 Nov;35(11):2918-2922.
Andersen, Anders P. ; Elliott, David A. ; Lawson, Moira ; Barland, Peter ; Hatcher, Victor Bernard ; Puszkin, Elena G. / Growth and morphological transformations of Helicobacter pylori in broth media. In: Journal of Clinical Microbiology. 1997 ; Vol. 35, No. 11. pp. 2918-2922.
@article{74c397c8096d4560be3cadbc47289fa2,
title = "Growth and morphological transformations of Helicobacter pylori in broth media",
abstract = "Helicobacter pylori, a cause of peptic ulcer disease and certain types of gastric cancers, has usually been cultured on diverse agar-based media, resulting in a requirement for 2 to 4 days of growth at 37°C. We have developed a novel broth medium consisting of a base medium supplemented with 2{\%} newborn calf serum, Mg2+, Cu2+, Fe2+, Zn2+, Mn2+, and 1 mg of lysed human erythrocytes per ml. This medium supports rapid growth of H. pylori, with a doubling time of about 50 min. Optimal growth was obtained in a pH range higher than that supporting most other gram-negative bacteria (at pH 8.5). H. pylori cultured in this supplemented broth retains the spiral morphology seen in both histological sections and cultures from agar-based media and also retains a high urease activity. After 18 h in this broth, H. pylori transforms to a coccal form with a complete loss of urease activity. Previously these cocci have been reported to be senescent, since they could not be subcultured on agar medium. Our experiments suggest that some of the cocci can revert back to the spiral morphology with full recovery of urease activity when subcultured in fresh microaerobic broth medium.",
author = "Andersen, {Anders P.} and Elliott, {David A.} and Moira Lawson and Peter Barland and Hatcher, {Victor Bernard} and Puszkin, {Elena G.}",
year = "1997",
month = "11",
language = "English (US)",
volume = "35",
pages = "2918--2922",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "11",

}

TY - JOUR

T1 - Growth and morphological transformations of Helicobacter pylori in broth media

AU - Andersen, Anders P.

AU - Elliott, David A.

AU - Lawson, Moira

AU - Barland, Peter

AU - Hatcher, Victor Bernard

AU - Puszkin, Elena G.

PY - 1997/11

Y1 - 1997/11

N2 - Helicobacter pylori, a cause of peptic ulcer disease and certain types of gastric cancers, has usually been cultured on diverse agar-based media, resulting in a requirement for 2 to 4 days of growth at 37°C. We have developed a novel broth medium consisting of a base medium supplemented with 2% newborn calf serum, Mg2+, Cu2+, Fe2+, Zn2+, Mn2+, and 1 mg of lysed human erythrocytes per ml. This medium supports rapid growth of H. pylori, with a doubling time of about 50 min. Optimal growth was obtained in a pH range higher than that supporting most other gram-negative bacteria (at pH 8.5). H. pylori cultured in this supplemented broth retains the spiral morphology seen in both histological sections and cultures from agar-based media and also retains a high urease activity. After 18 h in this broth, H. pylori transforms to a coccal form with a complete loss of urease activity. Previously these cocci have been reported to be senescent, since they could not be subcultured on agar medium. Our experiments suggest that some of the cocci can revert back to the spiral morphology with full recovery of urease activity when subcultured in fresh microaerobic broth medium.

AB - Helicobacter pylori, a cause of peptic ulcer disease and certain types of gastric cancers, has usually been cultured on diverse agar-based media, resulting in a requirement for 2 to 4 days of growth at 37°C. We have developed a novel broth medium consisting of a base medium supplemented with 2% newborn calf serum, Mg2+, Cu2+, Fe2+, Zn2+, Mn2+, and 1 mg of lysed human erythrocytes per ml. This medium supports rapid growth of H. pylori, with a doubling time of about 50 min. Optimal growth was obtained in a pH range higher than that supporting most other gram-negative bacteria (at pH 8.5). H. pylori cultured in this supplemented broth retains the spiral morphology seen in both histological sections and cultures from agar-based media and also retains a high urease activity. After 18 h in this broth, H. pylori transforms to a coccal form with a complete loss of urease activity. Previously these cocci have been reported to be senescent, since they could not be subcultured on agar medium. Our experiments suggest that some of the cocci can revert back to the spiral morphology with full recovery of urease activity when subcultured in fresh microaerobic broth medium.

UR - http://www.scopus.com/inward/record.url?scp=0030774796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030774796&partnerID=8YFLogxK

M3 - Article

VL - 35

SP - 2918

EP - 2922

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 11

ER -